TY - JOUR
T1 - Inhibition of telomerase activity by a cell-penetrating peptide nucleic acid construct in human melanoma cells
AU - Villa, Raffaella
AU - Folini, Marco
AU - Lualdi, Susanna
AU - Veronese, Silvio
AU - Daidone, Maria Grazia
AU - Zaffaroni, Nadia
PY - 2000/5/12
Y1 - 2000/5/12
N2 - We investigated the effect of two peptide nucleic acids (PNAs), which are complementary to the RNA component of human telomerase, on the catalytic activity of the enzyme. PNAs induced a dose-dependent reduction of telomerase activity in cell extracts from human melanoma cell lines and surgical specimens. To down-regulate telomerase in intact cells, we generated a chimeric molecule synthesized by coupling the 13-mer PNA to the Antennapedia peptide. The PNA construct induced a dose- and time-dependent inhibition of telomerase activity. However, a 20-day exposure to the PNA construct only caused a slight increase in melanoma cell doubling time and failed to induce any telomere shortening. (C) 2000 Federation of European Biochemical Societies.
AB - We investigated the effect of two peptide nucleic acids (PNAs), which are complementary to the RNA component of human telomerase, on the catalytic activity of the enzyme. PNAs induced a dose-dependent reduction of telomerase activity in cell extracts from human melanoma cell lines and surgical specimens. To down-regulate telomerase in intact cells, we generated a chimeric molecule synthesized by coupling the 13-mer PNA to the Antennapedia peptide. The PNA construct induced a dose- and time-dependent inhibition of telomerase activity. However, a 20-day exposure to the PNA construct only caused a slight increase in melanoma cell doubling time and failed to induce any telomere shortening. (C) 2000 Federation of European Biochemical Societies.
KW - Melanoma cell
KW - Peptide nucleic acid
KW - Telomerase
KW - Telomere
UR - http://www.scopus.com/inward/record.url?scp=0034640463&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034640463&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(00)01540-4
DO - 10.1016/S0014-5793(00)01540-4
M3 - Article
C2 - 10812083
AN - SCOPUS:0034640463
VL - 473
SP - 241
EP - 248
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -