Inhibition of telomerase activity by a cell-penetrating peptide nucleic acid construct in human melanoma cells

Raffaella Villa, Marco Folini, Susanna Lualdi, Silvio Veronese, Maria Grazia Daidone, Nadia Zaffaroni

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the effect of two peptide nucleic acids (PNAs), which are complementary to the RNA component of human telomerase, on the catalytic activity of the enzyme. PNAs induced a dose-dependent reduction of telomerase activity in cell extracts from human melanoma cell lines and surgical specimens. To down-regulate telomerase in intact cells, we generated a chimeric molecule synthesized by coupling the 13-mer PNA to the Antennapedia peptide. The PNA construct induced a dose- and time-dependent inhibition of telomerase activity. However, a 20-day exposure to the PNA construct only caused a slight increase in melanoma cell doubling time and failed to induce any telomere shortening. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalFEBS Letters
Volume473
Issue number2
DOIs
Publication statusPublished - May 12 2000

Keywords

  • Melanoma cell
  • Peptide nucleic acid
  • Telomerase
  • Telomere

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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