Inhibition of telomerase activity by a cell-penetrating peptide nucleic acid construct in human melanoma cells

Raffaella Villa; Marco Folini; Susanna Lualdi; Silvio Veronese; Maria Grazia Daidone; Nadia Zaffaroni

doi:10.1016/S0014-5793(00)01540-4

Inhibition of telomerase activity by a cell-penetrating peptide nucleic acid construct in human melanoma cells

Raffaella Villa, Marco Folini, Susanna Lualdi, Silvio Veronese, Maria Grazia Daidone, Nadia Zaffaroni

Fondazione IRCCS Istituto Nazionale dei Tumori

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated the effect of two peptide nucleic acids (PNAs), which are complementary to the RNA component of human telomerase, on the catalytic activity of the enzyme. PNAs induced a dose-dependent reduction of telomerase activity in cell extracts from human melanoma cell lines and surgical specimens. To down-regulate telomerase in intact cells, we generated a chimeric molecule synthesized by coupling the 13-mer PNA to the Antennapedia peptide. The PNA construct induced a dose- and time-dependent inhibition of telomerase activity. However, a 20-day exposure to the PNA construct only caused a slight increase in melanoma cell doubling time and failed to induce any telomere shortening. (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	241-248
Number of pages	8
Journal	FEBS Letters
Volume	473
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(00)01540-4
Publication status	Published - May 12 2000

Keywords

Melanoma cell
Peptide nucleic acid
Telomerase
Telomere

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/S0014-5793(00)01540-4

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abstract = "We investigated the effect of two peptide nucleic acids (PNAs), which are complementary to the RNA component of human telomerase, on the catalytic activity of the enzyme. PNAs induced a dose-dependent reduction of telomerase activity in cell extracts from human melanoma cell lines and surgical specimens. To down-regulate telomerase in intact cells, we generated a chimeric molecule synthesized by coupling the 13-mer PNA to the Antennapedia peptide. The PNA construct induced a dose- and time-dependent inhibition of telomerase activity. However, a 20-day exposure to the PNA construct only caused a slight increase in melanoma cell doubling time and failed to induce any telomere shortening. (C) 2000 Federation of European Biochemical Societies.",

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AU - Villa, Raffaella

AU - Folini, Marco

AU - Lualdi, Susanna

AU - Veronese, Silvio

AU - Daidone, Maria Grazia

AU - Zaffaroni, Nadia

PY - 2000/5/12

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AB - We investigated the effect of two peptide nucleic acids (PNAs), which are complementary to the RNA component of human telomerase, on the catalytic activity of the enzyme. PNAs induced a dose-dependent reduction of telomerase activity in cell extracts from human melanoma cell lines and surgical specimens. To down-regulate telomerase in intact cells, we generated a chimeric molecule synthesized by coupling the 13-mer PNA to the Antennapedia peptide. The PNA construct induced a dose- and time-dependent inhibition of telomerase activity. However, a 20-day exposure to the PNA construct only caused a slight increase in melanoma cell doubling time and failed to induce any telomere shortening. (C) 2000 Federation of European Biochemical Societies.

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KW - Peptide nucleic acid

KW - Telomerase

KW - Telomere

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Inhibition of telomerase activity by a cell-penetrating peptide nucleic acid construct in human melanoma cells

Abstract

Keywords

ASJC Scopus subject areas

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