Inhibition of the protease of human immunodeficiency virus blocks replication and infectivity of the virus in chronically infected macrophages

C. F. Perno, A. Bergamini, C. D. Pesce, G. Milanese, M. Capozzi, S. Aquaro, S. Thaisrivongs, W. G. Tarpley, G. Zon, C. D'Agostini, G. Rocchi, E. Garaci, R. Calio

Research output: Contribution to journalArticle

Abstract

Because of the importance of monocytes/macrophages (M/M) as an in vivo reservoir of human immunodeficiency virus (HIV), a study was done to investigate whether viral replication in chronically infected macrophages (HIV M/M) could be inhibited by various drugs, including U-75875, an inhibitor of HIV protease. HIV replication in M/M and in chronically infected T cells was dramatically decreased by U-75875, while other drugs, including zidovudine, interferon-α, and an antisense oligodeoxynucleotide against the rev gene, were effective antiviral agents only in de novo-infected cells. Virus titer in HIV M/M was reduced ~105-fold by nontoxic concentrations of U-75875, while no effect on HIV DNA or virus antigen expression on cell membrane was achieved in M/M infected either chronically or de novo. Thus, U- 75875 essentially worked against late stages of viral replication. These data support the use of protease inhibitors, alone or in combination, in the therapy of HIV-infected patients.

Original languageEnglish
Pages (from-to)1148-1156
Number of pages9
JournalJournal of Infectious Diseases
Volume168
Issue number5
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

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    Perno, C. F., Bergamini, A., Pesce, C. D., Milanese, G., Capozzi, M., Aquaro, S., Thaisrivongs, S., Tarpley, W. G., Zon, G., D'Agostini, C., Rocchi, G., Garaci, E., & Calio, R. (1993). Inhibition of the protease of human immunodeficiency virus blocks replication and infectivity of the virus in chronically infected macrophages. Journal of Infectious Diseases, 168(5), 1148-1156.