Inhibition of the SH3 domain-mediated binding of Src to the androgen receptor and its effect on tumor growth

A. Migliaccio, L. Varricchio, A. De Falco, G. Castoria, C. Arra, H. Yamaguchi, A. Ciociola, M. Lombardi, R. Di Stasio, A. Barbieri, A. Baldi, M. V. Barone, E. Appella, F. Auricchio

Research output: Contribution to journalArticle

Abstract

In human mammary and prostate cancer cells, steroid hormones or epidermal growth factor (EGF) trigger association of the androgen receptor (AR)-estradiol receptor (ER) (α or β) complex with Src. This interaction activates Src and affects the G1 to S cell cycle progression. In this report, we identify the sequence responsible for the AR/Src interaction and describe a 10 amino-acid peptide that inhibits this interaction. Treatment of the human prostate or mammary cancer cells (LNCaP or MCF-7, respectively) with nanomolar concentrations of this peptide inhibits the androgen- or estradiol-induced association between the AR or the ER and Src the Src/Erk pathway activation, cyclin D1 expression and DNA synthesis, without interfering in the receptor-dependent transcriptional activity. Similarly, the peptide prevents the S phase entry of LNCaP and MCF-7 cells treated with EGF as well as mouse embryo fibroblasts stimulated with androgen or EGF. Interestingly, the peptide does not inhibit the S phase entry and cytoskeletal changes induced by EGF or serum treatment of AR-negative prostate cancer cell lines. The peptide is the first example of a specific inhibitor of steroid receptor-dependent signal transducing activity. The importance of these results is highlighted by the finding that the peptide strongly inhibits the growth of LNCaP xenografts established in nude mice.

Original languageEnglish
Pages (from-to)6619-6629
Number of pages11
JournalOncogene
Volume26
Issue number46
DOIs
Publication statusPublished - Oct 11 2007

Keywords

  • Androgen receptor
  • Estrogen receptor
  • Receptor antagonist
  • Src
  • Xenografts

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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  • Cite this

    Migliaccio, A., Varricchio, L., De Falco, A., Castoria, G., Arra, C., Yamaguchi, H., Ciociola, A., Lombardi, M., Di Stasio, R., Barbieri, A., Baldi, A., Barone, M. V., Appella, E., & Auricchio, F. (2007). Inhibition of the SH3 domain-mediated binding of Src to the androgen receptor and its effect on tumor growth. Oncogene, 26(46), 6619-6629. https://doi.org/10.1038/sj.onc.1210487