Inhibition of the SUMO pathway by Gam1

Mariaelena Pozzebon, Chiara V. Segré, Susanna Chiocca

Research output: Chapter in Book/Report/Conference proceedingChapter


We have previously demonstrated that Gam1, an avian adenoviral protein inhibits sumoylation. By counteracting the SUMO pathway, Gam1 has a significant impact on virus-infected cells, but in isolation the inhibitory effects of the Gam1 protein can be exploited to intentionally manipulate the SUMO system in vivo or in vitro. Here we discuss in detail the techniques we use to inhibit the SUMO pathway using the Gam1 protein.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages17
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Degradation
  • Gam1
  • Inhibition
  • SAE1/SAE2
  • SUMO
  • SUMO E1
  • SUMO E2
  • Sumoylation
  • UBC9

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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