Inhibitor-3 ensures bipolar mitotic spindle attachment by limiting association of SDS22 with kinetochore-bound protein phosphatase-1

Annika Eiteneuer, Jonas Seiler, Matthias Weith, Monique Beullens, Bart Lesage, Veronica Krenn, Andrea Musacchio, Mathieu Bollen, Hemmo Meyer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Faithful chromosome segregation during mitosis is tightly regulated by opposing activities of Aurora B kinase and protein phosphatase-1 (PP1). PP1 function at kinetochores has been linked to SDS22, but the exact localization of SDS22 and how it affects PP1 are controversial. Here, we confirm that SDS22 is required for PP1 activity, but show that SDS22 does not normally localize to kinetochores. Instead, SDS22 is kept in solution by formation of a ternary complex with PP1 and inhibitor-3 (I3). Depletion of I3 does not affect the amount of PP1 at kinetochores but causes quantitative association of SDS22 with PP1 on KNL1 at the kinetochore. Such accumulation of SDS22 at kinetochores interferes with PP1 activity and inhibits Aurora B threonine-232 dephosphorylation, which leads to increased Aurora B activity in metaphase and persistence in anaphase accompanied with segregation defects. We propose a model in which I3 regulates an SDS22-mediated PP1 activation step in solution that precedes SDS22 dissociation and transfer of PP1 to kinetochores, and which is required for PP1 to efficiently antagonize Aurora B. Synopsis Reversible protein phosphorylation at kinetochores is essential for ensuring correct spindle attachment and chromosome segregation. Antagonism of Aurora B kinase action by protein phosphatase-1 is controlled by intricate interplay of two PP1-interacting regulators. SDS22 is essential to activate KNL1-bound PP1 at the kinetochore so that it can antagonize Aurora B during mitotic chromosome biorientation. SDS22 itself does not normally localize to kinetochores and increased SDS22 binding to PP1 at the kinetochore in fact inhibits PP1 activity. Inhibitor-3 (I3) forms a complex with SDS22-PP1 in solution and prevents association of SDS22 to KNL1-bound PP1 to ensure PP1 activity at the kinetochore. SDS22 in cooperation with I3 may act as a chaperone that activates PP1 in solution prior to recruitment to the kinetochore. Intricate interplay of two PP1-interacting regulators controls Aurora B-mediated kinetochore phosphorylation to ensure proper chromosome segregation.

Original languageEnglish
Pages (from-to)2704-2720
Number of pages17
JournalEMBO Journal
Volume33
Issue number22
DOIs
Publication statusPublished - Nov 18 2014

Fingerprint

Protein Phosphatase 1
Kinetochores
Spindle Apparatus
Association reactions
Chromosomes
Chromosome Segregation
Aurora Kinase B
Phosphorylation
Ternary Complex Factors

Keywords

  • Aurora B
  • chromosome segregation
  • kinetochore
  • mitosis
  • protein phosphatase-1

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Inhibitor-3 ensures bipolar mitotic spindle attachment by limiting association of SDS22 with kinetochore-bound protein phosphatase-1. / Eiteneuer, Annika; Seiler, Jonas; Weith, Matthias; Beullens, Monique; Lesage, Bart; Krenn, Veronica; Musacchio, Andrea; Bollen, Mathieu; Meyer, Hemmo.

In: EMBO Journal, Vol. 33, No. 22, 18.11.2014, p. 2704-2720.

Research output: Contribution to journalArticle

Eiteneuer, A, Seiler, J, Weith, M, Beullens, M, Lesage, B, Krenn, V, Musacchio, A, Bollen, M & Meyer, H 2014, 'Inhibitor-3 ensures bipolar mitotic spindle attachment by limiting association of SDS22 with kinetochore-bound protein phosphatase-1', EMBO Journal, vol. 33, no. 22, pp. 2704-2720. https://doi.org/10.15252/embj.201489054
Eiteneuer, Annika ; Seiler, Jonas ; Weith, Matthias ; Beullens, Monique ; Lesage, Bart ; Krenn, Veronica ; Musacchio, Andrea ; Bollen, Mathieu ; Meyer, Hemmo. / Inhibitor-3 ensures bipolar mitotic spindle attachment by limiting association of SDS22 with kinetochore-bound protein phosphatase-1. In: EMBO Journal. 2014 ; Vol. 33, No. 22. pp. 2704-2720.
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abstract = "Faithful chromosome segregation during mitosis is tightly regulated by opposing activities of Aurora B kinase and protein phosphatase-1 (PP1). PP1 function at kinetochores has been linked to SDS22, but the exact localization of SDS22 and how it affects PP1 are controversial. Here, we confirm that SDS22 is required for PP1 activity, but show that SDS22 does not normally localize to kinetochores. Instead, SDS22 is kept in solution by formation of a ternary complex with PP1 and inhibitor-3 (I3). Depletion of I3 does not affect the amount of PP1 at kinetochores but causes quantitative association of SDS22 with PP1 on KNL1 at the kinetochore. Such accumulation of SDS22 at kinetochores interferes with PP1 activity and inhibits Aurora B threonine-232 dephosphorylation, which leads to increased Aurora B activity in metaphase and persistence in anaphase accompanied with segregation defects. We propose a model in which I3 regulates an SDS22-mediated PP1 activation step in solution that precedes SDS22 dissociation and transfer of PP1 to kinetochores, and which is required for PP1 to efficiently antagonize Aurora B. Synopsis Reversible protein phosphorylation at kinetochores is essential for ensuring correct spindle attachment and chromosome segregation. Antagonism of Aurora B kinase action by protein phosphatase-1 is controlled by intricate interplay of two PP1-interacting regulators. SDS22 is essential to activate KNL1-bound PP1 at the kinetochore so that it can antagonize Aurora B during mitotic chromosome biorientation. SDS22 itself does not normally localize to kinetochores and increased SDS22 binding to PP1 at the kinetochore in fact inhibits PP1 activity. Inhibitor-3 (I3) forms a complex with SDS22-PP1 in solution and prevents association of SDS22 to KNL1-bound PP1 to ensure PP1 activity at the kinetochore. SDS22 in cooperation with I3 may act as a chaperone that activates PP1 in solution prior to recruitment to the kinetochore. Intricate interplay of two PP1-interacting regulators controls Aurora B-mediated kinetochore phosphorylation to ensure proper chromosome segregation.",
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AU - Lesage, Bart

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AU - Meyer, Hemmo

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N2 - Faithful chromosome segregation during mitosis is tightly regulated by opposing activities of Aurora B kinase and protein phosphatase-1 (PP1). PP1 function at kinetochores has been linked to SDS22, but the exact localization of SDS22 and how it affects PP1 are controversial. Here, we confirm that SDS22 is required for PP1 activity, but show that SDS22 does not normally localize to kinetochores. Instead, SDS22 is kept in solution by formation of a ternary complex with PP1 and inhibitor-3 (I3). Depletion of I3 does not affect the amount of PP1 at kinetochores but causes quantitative association of SDS22 with PP1 on KNL1 at the kinetochore. Such accumulation of SDS22 at kinetochores interferes with PP1 activity and inhibits Aurora B threonine-232 dephosphorylation, which leads to increased Aurora B activity in metaphase and persistence in anaphase accompanied with segregation defects. We propose a model in which I3 regulates an SDS22-mediated PP1 activation step in solution that precedes SDS22 dissociation and transfer of PP1 to kinetochores, and which is required for PP1 to efficiently antagonize Aurora B. Synopsis Reversible protein phosphorylation at kinetochores is essential for ensuring correct spindle attachment and chromosome segregation. Antagonism of Aurora B kinase action by protein phosphatase-1 is controlled by intricate interplay of two PP1-interacting regulators. SDS22 is essential to activate KNL1-bound PP1 at the kinetochore so that it can antagonize Aurora B during mitotic chromosome biorientation. SDS22 itself does not normally localize to kinetochores and increased SDS22 binding to PP1 at the kinetochore in fact inhibits PP1 activity. Inhibitor-3 (I3) forms a complex with SDS22-PP1 in solution and prevents association of SDS22 to KNL1-bound PP1 to ensure PP1 activity at the kinetochore. SDS22 in cooperation with I3 may act as a chaperone that activates PP1 in solution prior to recruitment to the kinetochore. Intricate interplay of two PP1-interacting regulators controls Aurora B-mediated kinetochore phosphorylation to ensure proper chromosome segregation.

AB - Faithful chromosome segregation during mitosis is tightly regulated by opposing activities of Aurora B kinase and protein phosphatase-1 (PP1). PP1 function at kinetochores has been linked to SDS22, but the exact localization of SDS22 and how it affects PP1 are controversial. Here, we confirm that SDS22 is required for PP1 activity, but show that SDS22 does not normally localize to kinetochores. Instead, SDS22 is kept in solution by formation of a ternary complex with PP1 and inhibitor-3 (I3). Depletion of I3 does not affect the amount of PP1 at kinetochores but causes quantitative association of SDS22 with PP1 on KNL1 at the kinetochore. Such accumulation of SDS22 at kinetochores interferes with PP1 activity and inhibits Aurora B threonine-232 dephosphorylation, which leads to increased Aurora B activity in metaphase and persistence in anaphase accompanied with segregation defects. We propose a model in which I3 regulates an SDS22-mediated PP1 activation step in solution that precedes SDS22 dissociation and transfer of PP1 to kinetochores, and which is required for PP1 to efficiently antagonize Aurora B. Synopsis Reversible protein phosphorylation at kinetochores is essential for ensuring correct spindle attachment and chromosome segregation. Antagonism of Aurora B kinase action by protein phosphatase-1 is controlled by intricate interplay of two PP1-interacting regulators. SDS22 is essential to activate KNL1-bound PP1 at the kinetochore so that it can antagonize Aurora B during mitotic chromosome biorientation. SDS22 itself does not normally localize to kinetochores and increased SDS22 binding to PP1 at the kinetochore in fact inhibits PP1 activity. Inhibitor-3 (I3) forms a complex with SDS22-PP1 in solution and prevents association of SDS22 to KNL1-bound PP1 to ensure PP1 activity at the kinetochore. SDS22 in cooperation with I3 may act as a chaperone that activates PP1 in solution prior to recruitment to the kinetochore. Intricate interplay of two PP1-interacting regulators controls Aurora B-mediated kinetochore phosphorylation to ensure proper chromosome segregation.

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