Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line

Jocelyn Vedrenne, Eric Assier, Raffaele Pereno, Haniaa Bouzinba-Segard, Bruno Azzarone, Claude Jasmin, Dominique Charron, Patricia Krief

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Abstract

The expression of major histocompatibility complex (MHC) class II antigens is constitutive in professional antigen presenting cells (APCs) but can also be induced by interferon-gamma (IFN-γ) on the majority of the non professional APCs (e.g. fibroblasts). We have recently characterised a new factor called IK which is an efficient inhibitor of IFN-γ induction of MHC class II antigens expression. Here, we demonstrate a novel role for IK in MHC class II expression since over-expression of this protein by stable transfection into human B cells led to a total disappearance of constitutive MHC class II mRNA expression. The class II transactivator (CIITA) is necessary for both constitutive and IFN-γ induced MHC class II expressions. Examination of CIITA mRNA in IK stably transfected clones revealed a marked reduction of CIITA mRNA transcription. Taken together these results demonstrate that the IK protein plays a key role in the constitutive expression of MHC class II antigens and that inhibition induced by IK is upstream of CIITA in this regulatory pathway.

Original languageEnglish
Pages (from-to)1453-1461
Number of pages9
JournalOncogene
Volume14
Issue number12
Publication statusPublished - 1997

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Keywords

  • CIITA
  • IFN-γ
  • IK
  • MHC class II

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Vedrenne, J., Assier, E., Pereno, R., Bouzinba-Segard, H., Azzarone, B., Jasmin, C., Charron, D., & Krief, P. (1997). Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line. Oncogene, 14(12), 1453-1461.