Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line

Jocelyn Vedrenne, Eric Assier, Raffaele Pereno, Haniaa Bouzinba-Segard, Bruno Azzarone, Claude Jasmin, Dominique Charron, Patricia Krief

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The expression of major histocompatibility complex (MHC) class II antigens is constitutive in professional antigen presenting cells (APCs) but can also be induced by interferon-gamma (IFN-γ) on the majority of the non professional APCs (e.g. fibroblasts). We have recently characterised a new factor called IK which is an efficient inhibitor of IFN-γ induction of MHC class II antigens expression. Here, we demonstrate a novel role for IK in MHC class II expression since over-expression of this protein by stable transfection into human B cells led to a total disappearance of constitutive MHC class II mRNA expression. The class II transactivator (CIITA) is necessary for both constitutive and IFN-γ induced MHC class II expressions. Examination of CIITA mRNA in IK stably transfected clones revealed a marked reduction of CIITA mRNA transcription. Taken together these results demonstrate that the IK protein plays a key role in the constitutive expression of MHC class II antigens and that inhibition induced by IK is upstream of CIITA in this regulatory pathway.

Original languageEnglish
Pages (from-to)1453-1461
Number of pages9
JournalOncogene
Volume14
Issue number12
Publication statusPublished - 1997

Fingerprint

Histocompatibility Antigens Class II
HLA Antigens
Major Histocompatibility Complex
Interferon-gamma
B-Lymphocytes
Cell Line
Antigen-Presenting Cells
Messenger RNA
Transfection
Proteins
Clone Cells
Fibroblasts
MHC class II transactivator protein

Keywords

  • CIITA
  • IFN-γ
  • IK
  • MHC class II

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Vedrenne, J., Assier, E., Pereno, R., Bouzinba-Segard, H., Azzarone, B., Jasmin, C., ... Krief, P. (1997). Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line. Oncogene, 14(12), 1453-1461.

Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line. / Vedrenne, Jocelyn; Assier, Eric; Pereno, Raffaele; Bouzinba-Segard, Haniaa; Azzarone, Bruno; Jasmin, Claude; Charron, Dominique; Krief, Patricia.

In: Oncogene, Vol. 14, No. 12, 1997, p. 1453-1461.

Research output: Contribution to journalArticle

Vedrenne, J, Assier, E, Pereno, R, Bouzinba-Segard, H, Azzarone, B, Jasmin, C, Charron, D & Krief, P 1997, 'Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line', Oncogene, vol. 14, no. 12, pp. 1453-1461.
Vedrenne J, Assier E, Pereno R, Bouzinba-Segard H, Azzarone B, Jasmin C et al. Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line. Oncogene. 1997;14(12):1453-1461.
Vedrenne, Jocelyn ; Assier, Eric ; Pereno, Raffaele ; Bouzinba-Segard, Haniaa ; Azzarone, Bruno ; Jasmin, Claude ; Charron, Dominique ; Krief, Patricia. / Inhibitor (IK) of IFN-γ induced HLA class II antigens expression also inhibits HLA class II constitutive expression in the human Raji B cell line. In: Oncogene. 1997 ; Vol. 14, No. 12. pp. 1453-1461.
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