Inhibitory activity of a synthetic pentapeptide on leukaemic myelopoiesis both in vitro and in vivo in rats

P. Foa, F. Chillemi, L. Lombardi, S. Lonati, A. T. Maiolo, E. E. Polli

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Abstract

The synthetic pentapeptide pGlu-Glu-Asp-Cys-Lys has recently been proposed as the active component of a granulocyte-derived inhibitor of normal haematopoiesis. We investigated its biological activity on leukaemic myelopoiesis both in vitro and in vivo in rats. Three different human permanent myeloid leukaemic cell lines (HL60, KG1 ML3) and a rat transplantable acute myeloid leukaemia (Shay leukaemia) were studied. Neither HL60 nor KG1 were sensitive to the peptide whereas a consistently reproducible inhibition of 3H-TdR uptake was observed in ML3 cells. This effect was not due to an unspecific toxic action on target cells and was spontaneously reversible. When injected i.p. twice daily at an appropriate concentration in rats bearing Shay leukaemia, the peptide caused a significant increase in survival. Our results therefore indicate that the synthetic pentapeptide studied inhibits not only normal but also leukaemic myelopoiesis.

Original languageEnglish
Pages (from-to)399-403
Number of pages5
JournalEuropean Journal of Haematology
Volume39
Issue number5
Publication statusPublished - 1987

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ASJC Scopus subject areas

  • Hematology

Cite this

Foa, P., Chillemi, F., Lombardi, L., Lonati, S., Maiolo, A. T., & Polli, E. E. (1987). Inhibitory activity of a synthetic pentapeptide on leukaemic myelopoiesis both in vitro and in vivo in rats. European Journal of Haematology, 39(5), 399-403.