Inhibitory effect of 17β-estradiol on growth and the polyamine metabolism of a human gastric carcinoma cell line (HGC-27)

C. Messa, M. Pricci, M. Linsalata, F. Russo, Alfredo Di Leo

Research output: Contribution to journalArticle

Abstract

Background: Estrogens may, by means of their receptors, modulate the growth of several tumors including gastrointestinal neoplasms. This control may occur through interaction with other molecules such as polyamines. An inverse relation between polyamine levels and the estrogen receptorial content has previously been demonstrated in vivo in human gastric carcinoma. The aim of the present study was to evaluate the effects of 17β-Estradiol administration on the in vitro cell proliferation rates and the polyamine metabolism of an estrogen receptor-positive human gastric cancer cell line (HGC-27). Methods: Estrogen receptors were detected with enzyme immunoassay. Cell proliferation was assessed by means of [3H]-thymidine incorporation and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. The polyamine content was evaluated with high-performance liquid chromatography and the ornithine decarboxylase activity with a radiometric technique. Results: Exposure of HGC-27 cells to increasing concentrations of 17β- Estradiol showed that an antiproliferative action became evident at concentrations of 8 μM or higher. At such concentrations, ornithine decarboxylase (ODC) activity was also significantly reduced, as were all polyamine levels, compared with the untreated control. These findings suggest that one of the mechanisms underlying 17β-Estradiol inhibition of HGC-27 cell proliferation is a decrease in ODC activity and, hence, in polyamine production.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalScandinavian Journal of Gastroenterology
Volume34
Issue number1
DOIs
Publication statusPublished - 1999

Keywords

  • 17β-Estradiol
  • HGC-27 cell line
  • Ornithine decarboxylase
  • Polyamines

ASJC Scopus subject areas

  • Gastroenterology

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