TY - JOUR
T1 - Inhibitory effect of HIV-1 envelope glycoproteins gp120 and gp160 on the in vitro growth of enriched (CD34+) hematopoietic progenitor cells
AU - Zauli, G.
AU - Re, Maria C.
AU - Visani, G.
AU - Furlini, G.
AU - La Placa, M.
PY - 1992/9
Y1 - 1992/9
N2 - The effect of increasing concentrations (from 0.01 to 10 μg/ml) of HIV-1 envelope glycoproteins gp160, gp120, gp41 and core protein p24 was evaluated on the in vitro growth of enriched hematopoietic progenitors (CD34+ cells). Both gp120 and gp160, at concentrations from 0.01 to 10 μg/ml, caused a progressive and significant (p+ cell count in liquid cultures supplemented with 2 ng/ml of human recombinant (r) interleukin-3 (IL-3), evaluated by means of Trypan-blue exclusion and [3H]thymidine ([3H]TdR) incorporation. In the absence of rIL-3, no inhibitory effects were observed even at the highest gp160 and gp120 concentrations explored (10 μg/ml). On the contrary, gp41 and p24 did not affect the number of viable CD34+ cells, either in the presence or in the absence of rIL-3. Moreover, gp160 and gp120, but not gp41 and p24, significantly (p+ cell surface, independently of an infectious process.
AB - The effect of increasing concentrations (from 0.01 to 10 μg/ml) of HIV-1 envelope glycoproteins gp160, gp120, gp41 and core protein p24 was evaluated on the in vitro growth of enriched hematopoietic progenitors (CD34+ cells). Both gp120 and gp160, at concentrations from 0.01 to 10 μg/ml, caused a progressive and significant (p+ cell count in liquid cultures supplemented with 2 ng/ml of human recombinant (r) interleukin-3 (IL-3), evaluated by means of Trypan-blue exclusion and [3H]thymidine ([3H]TdR) incorporation. In the absence of rIL-3, no inhibitory effects were observed even at the highest gp160 and gp120 concentrations explored (10 μg/ml). On the contrary, gp41 and p24 did not affect the number of viable CD34+ cells, either in the presence or in the absence of rIL-3. Moreover, gp160 and gp120, but not gp41 and p24, significantly (p+ cell surface, independently of an infectious process.
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U2 - 10.1007/BF01317189
DO - 10.1007/BF01317189
M3 - Article
C2 - 1370604
AN - SCOPUS:0026461619
VL - 122
SP - 271
EP - 280
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 3-4
ER -