Inhibitory effect of recombinant intracellular interleukin 1 receptor antagonist on endothelial cell activation

Riccardo Bertini, Marina Sironi, Ines Martin-Padura, Francesco Colotta, Sandro Rambaldi, Sergio Bernasconi, Pietro Ghezzi, Stephen J. Haskill, David Liu, Alberto Mantovani

Research output: Contribution to journalArticle

Abstract

This investigation was designed to elucidate whether an intracellular version of interleukin 1 receptor antagonist (icIL-1ra) interferes with the action of IL-1 at the level of vascular cells. Recombinant icIL-1ra inhibited the IL-1-induced production of IL-6, IL-8 and monocyte chemotactic protein by human endothelial cells (HEC). Moreover, icIL-1ra inhibited induction of adhesion molecules by IL-1. Endotoxin lipopolysaccharide (LPS), an IL-1 inducer, stimulated a spectrum of functions in EC similar to that activated by IL-1, but icIL-1ra did not interfere with the LPS activation of EC. This observation suggests that induction of extracellular IL-1 is not an important intermediate event in the response of EC to LPS. Unlike LPS-stimulated monocytes, EC exposed to different inducers did not express appreciable levels of IL-1ra mRNA transcripts as assessed by northern blot analysis. IL-1ra produced by mononuclear phagocytes, represents a negative regulator circuit of the action of IL-1 on EC and could be important in the control of vascular participation in inflammation and immunity.

Original languageEnglish
Pages (from-to)44-47
Number of pages4
JournalCytokine
Volume4
Issue number1
DOIs
Publication statusPublished - 1992

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Keywords

  • endothelial cells
  • inflammation
  • interleukin-1-receptor antagonist

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Bertini, R., Sironi, M., Martin-Padura, I., Colotta, F., Rambaldi, S., Bernasconi, S., Ghezzi, P., Haskill, S. J., Liu, D., & Mantovani, A. (1992). Inhibitory effect of recombinant intracellular interleukin 1 receptor antagonist on endothelial cell activation. Cytokine, 4(1), 44-47. https://doi.org/10.1016/1043-4666(92)90035-P