Inhibitory effects of fenofibrate on apoptosis and cell proliferation in human endothelial cells in high glucose

Michela Zanetti, Alessia Stocca, Barbara Dapas, Rossella Farra, Laura Uxa, Alessandra Bosutti, Rocco Barazzoni, Fleur Bossi, Carlo Giansante, Francesco Tedesco, Luigi Cattin, Gianfranco Guarnieri, Gabriele Grassi

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Abstract

Fenofibrate has beneficial effects on the progression and clinical emergence of atherosclerosis in normoglycemic and in diabetic patients. Given the involvement of endothelium in these processes, we speculated that fenofibrate may influence endothelial cell apoptosis and proliferation, regulators of endothelium integrity. Fenofibrate effects on apoptosis and proliferation were studied in human umbilical vein endothelial cells under normal (5.5 mmol/l, NG) and high (22 mmol/l, HG) glucose with or without fenofibrate (50 μmol/l). Apoptosis was evaluated by annexin V, by poly(ADP-ribose) polymerase protein cleavage, and cyclooxygenase-2 (COX-2), Bax/Bcl-2, and p53 protein levels; proliferation was assessed by determining cell cycle phase distribution and the amounts of the cell cycle regulators E2F1, cyclin D1, E1, and A and the levels of the hyper-phosphorylated form of the retinoblastoma protein (ppRb). HG resulted in increased (p

Original languageEnglish
Pages (from-to)185-195
Number of pages11
JournalJournal of Molecular Medicine
Volume86
Issue number2
DOIs
Publication statusPublished - Feb 2008

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Keywords

  • Apoptosis
  • Cell proliferation
  • Diabetes mellitus
  • Endothelium
  • Macrovascular disease

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Zanetti, M., Stocca, A., Dapas, B., Farra, R., Uxa, L., Bosutti, A., Barazzoni, R., Bossi, F., Giansante, C., Tedesco, F., Cattin, L., Guarnieri, G., & Grassi, G. (2008). Inhibitory effects of fenofibrate on apoptosis and cell proliferation in human endothelial cells in high glucose. Journal of Molecular Medicine, 86(2), 185-195. https://doi.org/10.1007/s00109-007-0257-3