Inhibitory influence of chromogranin A N-terminal fragment (vasostatin-1) on the spontaneous contractions of rat proximal colon

Antonella Amato, Angelo Corti, Rosa Serio, Flavia Mulè

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Very little is known about the role played by CGA and its fragments in the gastrointestinal physiology. We have studied the role of CGA N-terminal fragments in the regulation of intestinal smooth muscle contractility by measuring the influence of recombinant CGA 1-78 (VS-1) and synthetic CGA 7-57 peptides on the spontaneous mechanical activity of rat proximal colon in vitro. The mechanical activity was recorded as changes in the intraluminal pressure. VS-1 (0.1-30 nM) and CGA 7-57 (10-300 nM) produced concentration-dependent inhibitory effects, characterized by a progressive decrease in the mean amplitude of circular muscle spontaneous contractions, without affecting the resting tone. The response to VS-1 was antagonised by anti-CGA monoclonal antibodies (mAb5A8, B4E11, 7D1 or 4D5) but not by an irrelevant antibody, indicating that the effect was specific. The inhibitory responses to VS-1 and to CGA 7-57 were significantly reduced by pre-treatment of the preparations with Nω-nitro-l-arginine methyl ester (l-NAME) (300 μM), 1H-(1,2,4) oxadiazolo-(4,3-a) quinoxalin-1-one (ODQ) (10 μM), apamin (0.1 μM) or tetrodotoxin (TTX) (1 μM). The results suggest that VS-1 plays an inhibitory modulatory role on spontaneous contractions rat colon circular muscle, through mechanisms involving in part neural release of nitric oxide.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalRegulatory Peptides
Volume130
Issue number1-2
DOIs
Publication statusPublished - Aug 15 2005

Fingerprint

Chromogranin A
Muscle
Rats
Colon
Apamin
Physiology
Tetrodotoxin
Muscle Contraction
Smooth Muscle
Nitric Oxide
Monoclonal Antibodies
Pressure
Muscles
Peptides
Antibodies
vasostatin I
chromogranin A (7-57)

Keywords

  • CGA-derived peptides
  • Chromogranin A
  • Intestinal smooth muscle
  • Nitric oxide
  • Vasostatin-1

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Inhibitory influence of chromogranin A N-terminal fragment (vasostatin-1) on the spontaneous contractions of rat proximal colon. / Amato, Antonella; Corti, Angelo; Serio, Rosa; Mulè, Flavia.

In: Regulatory Peptides, Vol. 130, No. 1-2, 15.08.2005, p. 42-47.

Research output: Contribution to journalArticle

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