Injectable pectin hydrogels produced by internal gelation: PH dependence of gelling and rheological properties

Helena R. Moreira, Fabiola Munarin, Roberta Gentilini, Livia Visai, Pedro L. Granja, Maria Cristina Tanzi, Paola Petrini

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The production of injectable pectin hydrogels by internal gelation with calcium carbonate is proposed. The pH of pectin was increased with NaOH or NaHCO3 to reach physiological values. The determination of the equivalence point provided evidence that the pH can be more precisely modulated with NaHCO3 than with NaOH. Degradation and inability to gel was observed for pectin solutions with pH 5.35 or higher. Therefore, pectin solutions with pH values varying from 3.2 (native pH) to 3.8 were chosen to produce the gels. The increase of the pH for the crosslinked hydrogels, as well as the reduction of the gelling time and their thickening, was dependent upon the amount of calcium carbonate, as confirmed by rheology. Hydrogel extracts were not cytotoxic for L-929 fibroblasts. On the overall, the investigated formulations represent interesting injectable systems providing an adequate microenvironment for cell, drug or bioactive molecules delivery.

Original languageEnglish
Pages (from-to)339-347
Number of pages9
JournalCarbohydrate Polymers
Volume103
Issue number1
DOIs
Publication statusPublished - Mar 15 2014

Fingerprint

Hydrogels
Gelation
Calcium carbonate
Calcium Carbonate
Gels
Fibroblasts
Rheology
Hydrogel
Degradation
Molecules
pectin
Pharmaceutical Preparations

Keywords

  • Biomaterials
  • Internal gelation
  • Natural polymers
  • Pectin
  • Tissue engineering

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Injectable pectin hydrogels produced by internal gelation : PH dependence of gelling and rheological properties. / Moreira, Helena R.; Munarin, Fabiola; Gentilini, Roberta; Visai, Livia; Granja, Pedro L.; Tanzi, Maria Cristina; Petrini, Paola.

In: Carbohydrate Polymers, Vol. 103, No. 1, 15.03.2014, p. 339-347.

Research output: Contribution to journalArticle

Moreira, Helena R. ; Munarin, Fabiola ; Gentilini, Roberta ; Visai, Livia ; Granja, Pedro L. ; Tanzi, Maria Cristina ; Petrini, Paola. / Injectable pectin hydrogels produced by internal gelation : PH dependence of gelling and rheological properties. In: Carbohydrate Polymers. 2014 ; Vol. 103, No. 1. pp. 339-347.
@article{9cf32513fc484281b4f10d2c9cf124fc,
title = "Injectable pectin hydrogels produced by internal gelation: PH dependence of gelling and rheological properties",
abstract = "The production of injectable pectin hydrogels by internal gelation with calcium carbonate is proposed. The pH of pectin was increased with NaOH or NaHCO3 to reach physiological values. The determination of the equivalence point provided evidence that the pH can be more precisely modulated with NaHCO3 than with NaOH. Degradation and inability to gel was observed for pectin solutions with pH 5.35 or higher. Therefore, pectin solutions with pH values varying from 3.2 (native pH) to 3.8 were chosen to produce the gels. The increase of the pH for the crosslinked hydrogels, as well as the reduction of the gelling time and their thickening, was dependent upon the amount of calcium carbonate, as confirmed by rheology. Hydrogel extracts were not cytotoxic for L-929 fibroblasts. On the overall, the investigated formulations represent interesting injectable systems providing an adequate microenvironment for cell, drug or bioactive molecules delivery.",
keywords = "Biomaterials, Internal gelation, Natural polymers, Pectin, Tissue engineering",
author = "Moreira, {Helena R.} and Fabiola Munarin and Roberta Gentilini and Livia Visai and Granja, {Pedro L.} and Tanzi, {Maria Cristina} and Paola Petrini",
year = "2014",
month = "3",
day = "15",
doi = "10.1016/j.carbpol.2013.12.057",
language = "English",
volume = "103",
pages = "339--347",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Injectable pectin hydrogels produced by internal gelation

T2 - PH dependence of gelling and rheological properties

AU - Moreira, Helena R.

AU - Munarin, Fabiola

AU - Gentilini, Roberta

AU - Visai, Livia

AU - Granja, Pedro L.

AU - Tanzi, Maria Cristina

AU - Petrini, Paola

PY - 2014/3/15

Y1 - 2014/3/15

N2 - The production of injectable pectin hydrogels by internal gelation with calcium carbonate is proposed. The pH of pectin was increased with NaOH or NaHCO3 to reach physiological values. The determination of the equivalence point provided evidence that the pH can be more precisely modulated with NaHCO3 than with NaOH. Degradation and inability to gel was observed for pectin solutions with pH 5.35 or higher. Therefore, pectin solutions with pH values varying from 3.2 (native pH) to 3.8 were chosen to produce the gels. The increase of the pH for the crosslinked hydrogels, as well as the reduction of the gelling time and their thickening, was dependent upon the amount of calcium carbonate, as confirmed by rheology. Hydrogel extracts were not cytotoxic for L-929 fibroblasts. On the overall, the investigated formulations represent interesting injectable systems providing an adequate microenvironment for cell, drug or bioactive molecules delivery.

AB - The production of injectable pectin hydrogels by internal gelation with calcium carbonate is proposed. The pH of pectin was increased with NaOH or NaHCO3 to reach physiological values. The determination of the equivalence point provided evidence that the pH can be more precisely modulated with NaHCO3 than with NaOH. Degradation and inability to gel was observed for pectin solutions with pH 5.35 or higher. Therefore, pectin solutions with pH values varying from 3.2 (native pH) to 3.8 were chosen to produce the gels. The increase of the pH for the crosslinked hydrogels, as well as the reduction of the gelling time and their thickening, was dependent upon the amount of calcium carbonate, as confirmed by rheology. Hydrogel extracts were not cytotoxic for L-929 fibroblasts. On the overall, the investigated formulations represent interesting injectable systems providing an adequate microenvironment for cell, drug or bioactive molecules delivery.

KW - Biomaterials

KW - Internal gelation

KW - Natural polymers

KW - Pectin

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84892575017&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892575017&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2013.12.057

DO - 10.1016/j.carbpol.2013.12.057

M3 - Article

C2 - 24528738

AN - SCOPUS:84892575017

VL - 103

SP - 339

EP - 347

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 1

ER -