Injectable pectin hydrogels produced by internal gelation: PH dependence of gelling and rheological properties

Helena R. Moreira, Fabiola Munarin, Roberta Gentilini, Livia Visai, Pedro L. Granja, Maria Cristina Tanzi, Paola Petrini

Research output: Contribution to journalArticle

Abstract

The production of injectable pectin hydrogels by internal gelation with calcium carbonate is proposed. The pH of pectin was increased with NaOH or NaHCO3 to reach physiological values. The determination of the equivalence point provided evidence that the pH can be more precisely modulated with NaHCO3 than with NaOH. Degradation and inability to gel was observed for pectin solutions with pH 5.35 or higher. Therefore, pectin solutions with pH values varying from 3.2 (native pH) to 3.8 were chosen to produce the gels. The increase of the pH for the crosslinked hydrogels, as well as the reduction of the gelling time and their thickening, was dependent upon the amount of calcium carbonate, as confirmed by rheology. Hydrogel extracts were not cytotoxic for L-929 fibroblasts. On the overall, the investigated formulations represent interesting injectable systems providing an adequate microenvironment for cell, drug or bioactive molecules delivery.

Original languageEnglish
Pages (from-to)339-347
Number of pages9
JournalCarbohydrate Polymers
Volume103
Issue number1
DOIs
Publication statusPublished - Mar 15 2014

Keywords

  • Biomaterials
  • Internal gelation
  • Natural polymers
  • Pectin
  • Tissue engineering

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

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