Innate and adaptive immunity in self-reported nonceliac gluten sensitivity versus celiac disease

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13 Citations (Scopus)

Abstract

Background: Immune mechanisms have been implicated in nonceliac gluten sensitivity (NCGS), a condition characterized by intestinal and/or extraintestinal symptoms caused by the ingestion of gluten in non-celiac/non-wheat allergic individuals. Aims: We investigated innate and adaptive immunity in self-reported NCGS versus celiac disease (CD). Methods: In the supernatants of ex vivo-cultured duodenal biopsies from 14 self-reported NCGS patients, 9 untreated and 10 treated CD patients, and 12 controls we detected innate cytokines - interleukin (IL)-15, tumor necrosis factor-α, IL-1β, IL-6, IL-12p70, IL-23, IL-27, IL-32α, thymic stromal lymphopoietin (TSLP), IFN-α-, adaptive cytokines - interferon (IFN)-γ, IL-17A, IL-4, IL-5, IL-10, IL-13-, chemokines - IL-8, CCL1, CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL10-, granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Results: Mucosal innate and adaptive cytokines, chemokines and growth factors did not differ between self-reported NCGS, treated CD and controls. On the contrary, IL-6, IL-15, IL-27, IFN-α, IFN-γ, IL-17A, IL-23, G-CSF, GM-CSF, IL-8, CCL1 and CCL4 were significantly higher in untreated CD than in self-reported NCGS, treated CD and controls, while TSLP was significantly lower in untreated CD than in self-reported NCGS, treated CD and controls. Conclusion: In our hands, patients with self-reported NCGS showed no abnormalities of the mucosal immune response.

Original languageEnglish
JournalDigestive and Liver Disease
DOIs
Publication statusAccepted/In press - Dec 13 2015

Fingerprint

Glutens
Celiac Disease
Adaptive Immunity
Innate Immunity
Interferons
Interleukin-27
Interleukin-23
Interleukin-15
Interleukin-17
Interleukins
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Cytokines
Interleukin-8
Chemokines
Interleukin-6
Mucosal Immunity
Interleukin-13
Interleukin-5
Interleukin-1

Keywords

  • Chemokine
  • Cytokine
  • Duodenal mucosa
  • Tissue transglutaminase

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

@article{1dc182079b8247eebbe5b1d29ad42fb8,
title = "Innate and adaptive immunity in self-reported nonceliac gluten sensitivity versus celiac disease",
abstract = "Background: Immune mechanisms have been implicated in nonceliac gluten sensitivity (NCGS), a condition characterized by intestinal and/or extraintestinal symptoms caused by the ingestion of gluten in non-celiac/non-wheat allergic individuals. Aims: We investigated innate and adaptive immunity in self-reported NCGS versus celiac disease (CD). Methods: In the supernatants of ex vivo-cultured duodenal biopsies from 14 self-reported NCGS patients, 9 untreated and 10 treated CD patients, and 12 controls we detected innate cytokines - interleukin (IL)-15, tumor necrosis factor-α, IL-1β, IL-6, IL-12p70, IL-23, IL-27, IL-32α, thymic stromal lymphopoietin (TSLP), IFN-α-, adaptive cytokines - interferon (IFN)-γ, IL-17A, IL-4, IL-5, IL-10, IL-13-, chemokines - IL-8, CCL1, CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL10-, granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Results: Mucosal innate and adaptive cytokines, chemokines and growth factors did not differ between self-reported NCGS, treated CD and controls. On the contrary, IL-6, IL-15, IL-27, IFN-α, IFN-γ, IL-17A, IL-23, G-CSF, GM-CSF, IL-8, CCL1 and CCL4 were significantly higher in untreated CD than in self-reported NCGS, treated CD and controls, while TSLP was significantly lower in untreated CD than in self-reported NCGS, treated CD and controls. Conclusion: In our hands, patients with self-reported NCGS showed no abnormalities of the mucosal immune response.",
keywords = "Chemokine, Cytokine, Duodenal mucosa, Tissue transglutaminase",
author = "{Di Sabatino}, Antonio and Paolo Giuffrida and Giulia Fornasa and Chiara Salvatore and Alessandro Vanoli and Samuele Naviglio and {De Leo}, Luigina and Alessandra Pasini and {De Amici}, Mara and Costanza Alvisi and Tarcisio Not and Maria Rescigno and Corazza, {Gino Roberto}",
year = "2015",
month = "12",
day = "13",
doi = "10.1016/j.dld.2016.03.024",
language = "English",
journal = "Digestive and Liver Disease",
issn = "1590-8658",
publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Innate and adaptive immunity in self-reported nonceliac gluten sensitivity versus celiac disease

AU - Di Sabatino, Antonio

AU - Giuffrida, Paolo

AU - Fornasa, Giulia

AU - Salvatore, Chiara

AU - Vanoli, Alessandro

AU - Naviglio, Samuele

AU - De Leo, Luigina

AU - Pasini, Alessandra

AU - De Amici, Mara

AU - Alvisi, Costanza

AU - Not, Tarcisio

AU - Rescigno, Maria

AU - Corazza, Gino Roberto

PY - 2015/12/13

Y1 - 2015/12/13

N2 - Background: Immune mechanisms have been implicated in nonceliac gluten sensitivity (NCGS), a condition characterized by intestinal and/or extraintestinal symptoms caused by the ingestion of gluten in non-celiac/non-wheat allergic individuals. Aims: We investigated innate and adaptive immunity in self-reported NCGS versus celiac disease (CD). Methods: In the supernatants of ex vivo-cultured duodenal biopsies from 14 self-reported NCGS patients, 9 untreated and 10 treated CD patients, and 12 controls we detected innate cytokines - interleukin (IL)-15, tumor necrosis factor-α, IL-1β, IL-6, IL-12p70, IL-23, IL-27, IL-32α, thymic stromal lymphopoietin (TSLP), IFN-α-, adaptive cytokines - interferon (IFN)-γ, IL-17A, IL-4, IL-5, IL-10, IL-13-, chemokines - IL-8, CCL1, CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL10-, granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Results: Mucosal innate and adaptive cytokines, chemokines and growth factors did not differ between self-reported NCGS, treated CD and controls. On the contrary, IL-6, IL-15, IL-27, IFN-α, IFN-γ, IL-17A, IL-23, G-CSF, GM-CSF, IL-8, CCL1 and CCL4 were significantly higher in untreated CD than in self-reported NCGS, treated CD and controls, while TSLP was significantly lower in untreated CD than in self-reported NCGS, treated CD and controls. Conclusion: In our hands, patients with self-reported NCGS showed no abnormalities of the mucosal immune response.

AB - Background: Immune mechanisms have been implicated in nonceliac gluten sensitivity (NCGS), a condition characterized by intestinal and/or extraintestinal symptoms caused by the ingestion of gluten in non-celiac/non-wheat allergic individuals. Aims: We investigated innate and adaptive immunity in self-reported NCGS versus celiac disease (CD). Methods: In the supernatants of ex vivo-cultured duodenal biopsies from 14 self-reported NCGS patients, 9 untreated and 10 treated CD patients, and 12 controls we detected innate cytokines - interleukin (IL)-15, tumor necrosis factor-α, IL-1β, IL-6, IL-12p70, IL-23, IL-27, IL-32α, thymic stromal lymphopoietin (TSLP), IFN-α-, adaptive cytokines - interferon (IFN)-γ, IL-17A, IL-4, IL-5, IL-10, IL-13-, chemokines - IL-8, CCL1, CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL10-, granulocyte colony stimulating factor (G-CSF) and granulocyte-macrophage colony stimulating factor (GM-CSF). Results: Mucosal innate and adaptive cytokines, chemokines and growth factors did not differ between self-reported NCGS, treated CD and controls. On the contrary, IL-6, IL-15, IL-27, IFN-α, IFN-γ, IL-17A, IL-23, G-CSF, GM-CSF, IL-8, CCL1 and CCL4 were significantly higher in untreated CD than in self-reported NCGS, treated CD and controls, while TSLP was significantly lower in untreated CD than in self-reported NCGS, treated CD and controls. Conclusion: In our hands, patients with self-reported NCGS showed no abnormalities of the mucosal immune response.

KW - Chemokine

KW - Cytokine

KW - Duodenal mucosa

KW - Tissue transglutaminase

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U2 - 10.1016/j.dld.2016.03.024

DO - 10.1016/j.dld.2016.03.024

M3 - Article

AN - SCOPUS:84964607666

JO - Digestive and Liver Disease

JF - Digestive and Liver Disease

SN - 1590-8658

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