Restauración de la inmunidad innata en pacientes con infección por VIH/SIDA después de inicio de terapia antirretroviral

Translated title of the contribution: Innate immunity restoration in patients with HIV/AIDS infection associated with antiretroviral therapy

Alejandro Afani S, Lorena Jiusán L, Pablo Raby A, Giovanni Sitia, Javier Puente P, Cecilia Sepúlveda C, Dante Miranda W, Roy Cabrera C, Luca Guidotti, Paola Lanza

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Highly active antiretroviral therapy (HAART) in HIV/AIDS infection induces an important reduction of the viral load (VL) and an immune system reconstitution. CD4+ T lymphocyte count is the immunological measurement commonly used for the follow up of HIV/AIDS patients. Aim: To study prospectively the restoration of the innate immune system in patients with HIV/AIDS infection during their first year on HAART. Patients and Methods: 25 naive HIV/AIDS patients, from San José Hospital and University of Chile Clinical Hospital, Santiago, Chile, were studied between years 2002-2003. Every 4 months after HAART initiation, CD3+, CD4+, CD8+ T lymphocytes and CD16/56+ natural killer (NK) cells were quantified by flow cytometry. NK cell cytotoxicity was measured using radioactive chrome liberation (Cr51). Tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) were measured in peripheral blood mononuclear cells and viral load was determined using Amplicor HIV-1 from Roche Diagnostics Systems. Results: Thirteen of the 25 patients continued in the study. They were all males, average age 35 years old (23-50). At baseline average CD4+ count was 146 cells/μ L (31-362) and average viral load was 82.000 copies/mL (4.000-290.000). A raise in CD3+, CD4+, CD8+, and CD16/56 cells was noted at months 9-12 of therapy. Viral load became undetectable in the same period. NK cell function was decreased at the beginning of the therapy (1-4 months), reaching its highest values at months 9-12. There was no significant change in IL-10. TNF-α increased in six patients during the study. Conclusions: In this group of patients, innate immunity was restored during HAART. These results should be confirmed in studies with a longer follow up period and also measuring cytokines such as MIP-1α, MIP-1β and RANTES.

Translated title of the contributionInnate immunity restoration in patients with HIV/AIDS infection associated with antiretroviral therapy
Original languageSpanish
Pages (from-to)689-696
Number of pages8
JournalRevista Medica de Chile
Volume134
Issue number6
Publication statusPublished - 2006

Keywords

  • Antiretroviral therapy
  • CD4-positive T-lymphocytes
  • Highly active
  • HIV
  • Immunity
  • Natural

ASJC Scopus subject areas

  • Medicine(all)

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