Innate-like effector differentiation of human invariant NKT cells driven by IL-7

Claudia De Lalla, Nicola Festuccia, Inka Albrecht, Hyun Dong Chang, Grazia Andolfi, Ulrike Benninghoff, Ferdinando Bombelli, Giovanna Borsellino, Alessandro Aiuti, Andreas Radbruch, Paolo Dellabona, Giulia Casorati

Research output: Contribution to journalArticle

Abstract

Conventional MHC-restricted T lymphocytes leave thymus with a naive phenotype and require Ag-dependent stimulation coupled to proliferation to acquire effector functions. Invariant (i)NKT cells are a subset of T lymphocytes considered innate because they display an effector memory phenotype independent of TCR stimulation by foreign Ags. We investigated the effector differentiation program followed by human iNKT cells by studying cells from a relevant set of fetal thymi and umbilical cord blood samples. We find that human fetal iNKT cells have already started a differentiation program that activates the epigenetic and transcriptional control of ifng and il4 genes, leading at birth to cells that express these cytokines upon TCR signaling but independently of proliferation in vitro. Both ex vivo and in vitro analysis of fetal and neonatal iNKT cells delineate an effector differentiation program linked to cell division in vivo, and they identify IL-7 as one of the crucial signals driving this program in the apparent absence of Ag stimulation. Consistent with these data, human fetal and neonatal iNKT cells are hyperresponsive in vitro to IL-7 in comparison to conventional T cells, owing to an increased expression and signaling function of the IL-7 receptor α-chain. The innate nature of human iNKT cells could thus derive from lineage-specific developmental cues that selectively make these cells efficient IL-7 responders following thymic selection.

Original languageEnglish
Pages (from-to)4415-4424
Number of pages10
JournalJournal of Immunology
Volume180
Issue number7
Publication statusPublished - Apr 1 2008

ASJC Scopus subject areas

  • Immunology

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    De Lalla, C., Festuccia, N., Albrecht, I., Chang, H. D., Andolfi, G., Benninghoff, U., Bombelli, F., Borsellino, G., Aiuti, A., Radbruch, A., Dellabona, P., & Casorati, G. (2008). Innate-like effector differentiation of human invariant NKT cells driven by IL-7. Journal of Immunology, 180(7), 4415-4424.