Innovative composite HA scaffold rapid prototyping for bone reconstruction: An in-vitro pilot study

I. G. Lesci, L. Ciocca, B. Dozza, E. Lucarelli, S. Squarzoni, D. Donati, N. Roveri

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The ability to control the architecture and strength of a bone tissue engineering scaffold is critical to achieve a harmony between the scaffold and the host tissue. The scaffold attempts to mimic the function of the natural extracellular matrix, providing a temporary template for the growth of target tissues. The study of nanocrystalline calcium phosphate physical-chemical characteristics and, thereafter, the possibility to imitate bone mineral for the development of new advanced biomaterials is constantly growing. Scaffolds should have suitable architecture and strength to serve their intended function. Rapid prototyping (RP) technique is applied to tissue engineering to satisfy this need and to create a scaffold directly from the scanned and digitized image of the defect site. Design and construction of complex structures with different shapes and sizes, at micro and macro scale, with fully interconnected pore structure and appropriate mechanical properties are possible by using RP techniques. In this study we present a new biocompatible hybrid scaffold obtained through two different experimental methods and formed by synthetic biomimetic Hydroxyapatite (HA) nanocrystals with high surface reactivity which synergistically interacts with Poly(e-caprolactone) (PCL) and polylactic acid (PLLA). The aim of this pilot study is to test the adhesion and the proliferation of human mesenchymal stem cells (MSC) on both the scaffolds. MSC growth and distribution was evaluated 24 h and 7 days after in-vitro seeding. The results allowed the conclusion that these scaffolds are biocompatible and allow the colonization and proliferation of MSC, therefore, due to their mechanical properties, they are adequate for bone tissue engineering.

Original languageEnglish
Title of host publicationKey Engineering Materials
Pages56-63
Number of pages8
Volume583
DOIs
Publication statusPublished - 2014
Event5th International Conference on Biomaterials, Tissue Engineering and Medical Devices, BiomMedD 2012 - Constanta
Duration: Aug 29 2012Sep 1 2012

Publication series

NameKey Engineering Materials
Volume583
ISSN (Print)10139826

Other

Other5th International Conference on Biomaterials, Tissue Engineering and Medical Devices, BiomMedD 2012
CityConstanta
Period8/29/129/1/12

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Keywords

  • Biomaterial
  • Biomimetic hydroxyapatite
  • Bone scaffold
  • Rapid prototyping

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Lesci, I. G., Ciocca, L., Dozza, B., Lucarelli, E., Squarzoni, S., Donati, D., & Roveri, N. (2014). Innovative composite HA scaffold rapid prototyping for bone reconstruction: An in-vitro pilot study. In Key Engineering Materials (Vol. 583, pp. 56-63). (Key Engineering Materials; Vol. 583). https://doi.org/10.4028/www.scientific.net/KEM.583.56