Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases

Sara Marinelli, Valentina Vacca, Federica De Angelis, Luisa Pieroni, Tiziana Orsini, Chiara Parisi, Marzia Soligo, Virginia Protto, Luigi Manni, Roberto Guerrieri, Flaminia Pavone

Research output: Contribution to journalArticle

Abstract

Traumatic spinal cord injury has dramatic consequences and a huge social impact. We propose a new mouse model of spinal trauma that induces a complete paralysis of hindlimbs, still observable 30 days after injury. The contusion, performed without laminectomy and deriving from the pressure exerted directly on the bone, mimics more closely many features of spinal injury in humans. Spinal cord was injured at thoracic level 10 (T10) in adult anesthetized female CD1 mice, mounted on stereotaxic apparatus and connected to a precision impactor device. Following severe injury, we evaluated motor and sensory functions, and histological/morphological features of spinal tissue at different time points. Moreover, we studied the effects of early and subchronic administration of Docosahexaenoic acid, investigating functional responses, structural changes proximal and distal to the lesion in primary and secondary injury phases, proteome modulation in injured spinal cord. Docosahexaenoic acid was able i) to restore behavioural responses and ii) to induce pro-regenerative effects and neuroprotective action against demyelination, apoptosis and neuroinflammation. Considering the urgent health challenge represented by spinal injury, this new and reliable mouse model together with the positive effects of docosahexaenoic acid provide important translational implications for promising therapeutic approaches for spinal cord injuries.

Original languageEnglish
Article number8883
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

Fingerprint

Docosahexaenoic Acids
Spinal Cord Injuries
Spinal Injuries
Wounds and Injuries
Spinal Cord
Laminectomy
Contusions
Demyelinating Diseases
Proteome
Hindlimb
Social Change
Paralysis
Thorax
Apoptosis
Pressure
Bone and Bones
Equipment and Supplies
Health
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Innovative mouse model mimicking human-like features of spinal cord injury : efficacy of Docosahexaenoic acid on acute and chronic phases. / Marinelli, Sara; Vacca, Valentina; Angelis, Federica De; Pieroni, Luisa; Orsini, Tiziana; Parisi, Chiara; Soligo, Marzia; Protto, Virginia; Manni, Luigi; Guerrieri, Roberto; Pavone, Flaminia.

In: Scientific Reports, Vol. 9, No. 1, 8883, 01.12.2019.

Research output: Contribution to journalArticle

Marinelli, Sara ; Vacca, Valentina ; Angelis, Federica De ; Pieroni, Luisa ; Orsini, Tiziana ; Parisi, Chiara ; Soligo, Marzia ; Protto, Virginia ; Manni, Luigi ; Guerrieri, Roberto ; Pavone, Flaminia. / Innovative mouse model mimicking human-like features of spinal cord injury : efficacy of Docosahexaenoic acid on acute and chronic phases. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
@article{cce6097f634b4812b9fc15da67251c78,
title = "Innovative mouse model mimicking human-like features of spinal cord injury: efficacy of Docosahexaenoic acid on acute and chronic phases",
abstract = "Traumatic spinal cord injury has dramatic consequences and a huge social impact. We propose a new mouse model of spinal trauma that induces a complete paralysis of hindlimbs, still observable 30 days after injury. The contusion, performed without laminectomy and deriving from the pressure exerted directly on the bone, mimics more closely many features of spinal injury in humans. Spinal cord was injured at thoracic level 10 (T10) in adult anesthetized female CD1 mice, mounted on stereotaxic apparatus and connected to a precision impactor device. Following severe injury, we evaluated motor and sensory functions, and histological/morphological features of spinal tissue at different time points. Moreover, we studied the effects of early and subchronic administration of Docosahexaenoic acid, investigating functional responses, structural changes proximal and distal to the lesion in primary and secondary injury phases, proteome modulation in injured spinal cord. Docosahexaenoic acid was able i) to restore behavioural responses and ii) to induce pro-regenerative effects and neuroprotective action against demyelination, apoptosis and neuroinflammation. Considering the urgent health challenge represented by spinal injury, this new and reliable mouse model together with the positive effects of docosahexaenoic acid provide important translational implications for promising therapeutic approaches for spinal cord injuries.",
author = "Sara Marinelli and Valentina Vacca and Angelis, {Federica De} and Luisa Pieroni and Tiziana Orsini and Chiara Parisi and Marzia Soligo and Virginia Protto and Luigi Manni and Roberto Guerrieri and Flaminia Pavone",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41598-019-45037-x",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Innovative mouse model mimicking human-like features of spinal cord injury

T2 - efficacy of Docosahexaenoic acid on acute and chronic phases

AU - Marinelli, Sara

AU - Vacca, Valentina

AU - Angelis, Federica De

AU - Pieroni, Luisa

AU - Orsini, Tiziana

AU - Parisi, Chiara

AU - Soligo, Marzia

AU - Protto, Virginia

AU - Manni, Luigi

AU - Guerrieri, Roberto

AU - Pavone, Flaminia

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Traumatic spinal cord injury has dramatic consequences and a huge social impact. We propose a new mouse model of spinal trauma that induces a complete paralysis of hindlimbs, still observable 30 days after injury. The contusion, performed without laminectomy and deriving from the pressure exerted directly on the bone, mimics more closely many features of spinal injury in humans. Spinal cord was injured at thoracic level 10 (T10) in adult anesthetized female CD1 mice, mounted on stereotaxic apparatus and connected to a precision impactor device. Following severe injury, we evaluated motor and sensory functions, and histological/morphological features of spinal tissue at different time points. Moreover, we studied the effects of early and subchronic administration of Docosahexaenoic acid, investigating functional responses, structural changes proximal and distal to the lesion in primary and secondary injury phases, proteome modulation in injured spinal cord. Docosahexaenoic acid was able i) to restore behavioural responses and ii) to induce pro-regenerative effects and neuroprotective action against demyelination, apoptosis and neuroinflammation. Considering the urgent health challenge represented by spinal injury, this new and reliable mouse model together with the positive effects of docosahexaenoic acid provide important translational implications for promising therapeutic approaches for spinal cord injuries.

AB - Traumatic spinal cord injury has dramatic consequences and a huge social impact. We propose a new mouse model of spinal trauma that induces a complete paralysis of hindlimbs, still observable 30 days after injury. The contusion, performed without laminectomy and deriving from the pressure exerted directly on the bone, mimics more closely many features of spinal injury in humans. Spinal cord was injured at thoracic level 10 (T10) in adult anesthetized female CD1 mice, mounted on stereotaxic apparatus and connected to a precision impactor device. Following severe injury, we evaluated motor and sensory functions, and histological/morphological features of spinal tissue at different time points. Moreover, we studied the effects of early and subchronic administration of Docosahexaenoic acid, investigating functional responses, structural changes proximal and distal to the lesion in primary and secondary injury phases, proteome modulation in injured spinal cord. Docosahexaenoic acid was able i) to restore behavioural responses and ii) to induce pro-regenerative effects and neuroprotective action against demyelination, apoptosis and neuroinflammation. Considering the urgent health challenge represented by spinal injury, this new and reliable mouse model together with the positive effects of docosahexaenoic acid provide important translational implications for promising therapeutic approaches for spinal cord injuries.

UR - http://www.scopus.com/inward/record.url?scp=85067602528&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067602528&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-45037-x

DO - 10.1038/s41598-019-45037-x

M3 - Article

AN - SCOPUS:85067602528

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 8883

ER -