Inorganic polyphosphate and energy metabolism in mammalian cells

Evgeny Pavlov, Roozbeh Aschar-Sobbi, Michelangelo Campanella, Raymond J. Turner, María R. Gómez-García, Andrey Y. Abramov

Research output: Contribution to journalArticle

Abstract

Inorganic polyphosphate (poly P) is a polymer made from as few as 10 to several hundred phosphate molecules linked by phosphoanhydride bonds similar to ATP. Poly P is ubiquitous in all mammalian organisms, where it plays multiple physiological roles. The metabolism of poly P in mammalian organisms is not well understood. We have examined the mechanism of poly P production and the role of this polymer in cell energy metabolism. Poly P levels in mitochondria and intact cells were estimated using a fluorescent molecular probe, 4′,6-diamidino-2-phenylindole. Poly P levels were dependent on the metabolic state of the mitochondria. Poly P levels were increased by substrates of respiration and in turn reduced by mitochondrial inhibitor (rotenone) or an uncoupler (carbonylcyanidep-trifluoromethoxyphenylhydrazone). Oligomycin, an inhibitor of mitochondrial ATP-synthase, blocked the production of poly P. Enzymatic depletion of poly P from cells significantly altered the rate of ATP metabolism. We propose the existence of a feedback mechanism where poly P production and cell energy metabolism regulate each other.

Original languageEnglish
Pages (from-to)9420-9428
Number of pages9
JournalJournal of Biological Chemistry
Volume285
Issue number13
DOIs
Publication statusPublished - Mar 26 2010

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Polyphosphates
Energy Metabolism
Cells
Mitochondria
Metabolism
Polymers
Adenosine Triphosphate
Mitochondrial Proton-Translocating ATPases
Oligomycins
Molecular Probes
Rotenone
Fluorescent Dyes
Respiration
Phosphates
Feedback
Molecules
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Pavlov, E., Aschar-Sobbi, R., Campanella, M., Turner, R. J., Gómez-García, M. R., & Abramov, A. Y. (2010). Inorganic polyphosphate and energy metabolism in mammalian cells. Journal of Biological Chemistry, 285(13), 9420-9428. https://doi.org/10.1074/jbc.M109.013011

Inorganic polyphosphate and energy metabolism in mammalian cells. / Pavlov, Evgeny; Aschar-Sobbi, Roozbeh; Campanella, Michelangelo; Turner, Raymond J.; Gómez-García, María R.; Abramov, Andrey Y.

In: Journal of Biological Chemistry, Vol. 285, No. 13, 26.03.2010, p. 9420-9428.

Research output: Contribution to journalArticle

Pavlov, E, Aschar-Sobbi, R, Campanella, M, Turner, RJ, Gómez-García, MR & Abramov, AY 2010, 'Inorganic polyphosphate and energy metabolism in mammalian cells', Journal of Biological Chemistry, vol. 285, no. 13, pp. 9420-9428. https://doi.org/10.1074/jbc.M109.013011
Pavlov E, Aschar-Sobbi R, Campanella M, Turner RJ, Gómez-García MR, Abramov AY. Inorganic polyphosphate and energy metabolism in mammalian cells. Journal of Biological Chemistry. 2010 Mar 26;285(13):9420-9428. https://doi.org/10.1074/jbc.M109.013011
Pavlov, Evgeny ; Aschar-Sobbi, Roozbeh ; Campanella, Michelangelo ; Turner, Raymond J. ; Gómez-García, María R. ; Abramov, Andrey Y. / Inorganic polyphosphate and energy metabolism in mammalian cells. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 13. pp. 9420-9428.
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