Inosine-containing primers in human papillomavirus detection by polymerase chain reaction

G. Novelli; M. Gennarelli; L. De Santis; P. Aageloni; B. Dallapiccola

doi:10.1016/0753-3322(92)90025-3

Inosine-containing primers in human papillomavirus detection by polymerase chain reaction

G. Novelli, M. Gennarelli, L. De Santis, P. Aageloni, B. Dallapiccola

Research output: Contribution to journal › Article › peer-review

Abstract

A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV 16L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and is easy to use for HPV typing and screening. polymerase chain reaction / papillomavirus / inosine.

Original language	English
Pages (from-to)	167-169
Number of pages	3
Journal	Biomedicine and Pharmacotherapy
Volume	46
Issue number	4
DOIs	https://doi.org/10.1016/0753-3322(92)90025-3
Publication status	Published - 1992

ASJC Scopus subject areas

Pharmacology

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title = "Inosine-containing primers in human papillomavirus detection by polymerase chain reaction",

abstract = "A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV 16L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and is easy to use for HPV typing and screening. polymerase chain reaction / papillomavirus / inosine.",

author = "G. Novelli and M. Gennarelli and {De Santis}, L. and P. Aageloni and B. Dallapiccola",

year = "1992",

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AU - Novelli, G.

AU - Gennarelli, M.

AU - De Santis, L.

AU - Aageloni, P.

AU - Dallapiccola, B.

PY - 1992

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AB - A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV 16L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and is easy to use for HPV typing and screening. polymerase chain reaction / papillomavirus / inosine.

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