A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV 16L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and is easy to use for HPV typing and screening. polymerase chain reaction / papillomavirus / inosine.
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