Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons

Christopher A. Ross, Jacopo Meldolesi, Teresa A. Milner, Tomohide Satoh, Surachai Supattapone, Solomon H. Snyder

Research output: Contribution to journalArticle

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Abstract

INOSITOL 1,4,5-trisphosphate (InsP3) mediates the effects of several neurotransmitters, hormones and growth factors by mobilizing Ca2+ from a vesicular, non-mitochondrial intracellular store1. Many studies have indirectly suggested the endoplasmic reticulum (ER) to be the site of InsP3 action2-6, though some have implicated the plasma membrane7,8 or a newly described smooth surfaced structure, termed the calciosome9. Using antibodies directed against a purified InsP3-receptor glycoprotein10, of relative molecular mass 260,000, in electron microscope immunocytochemical studies of rat cerebellar Purkinje cells, we have now localized the InsP3 receptor to ER, including portions of the rough endoplasmic reticulum, a population of smooth-membrane-bound organelles (smooth ER), a portion of subplas-malemmal cisternae and the nuclear membrane, but not to mitochondria or the cell membrane. These results suggest that in cerebellar Purkinje cells, InsP3-induced intracellular calcium release is not the property of a single organelle, but is effected by specialized portions of both rough and smooth ER, and possibly by other smooth surfaced structures. The present findings are the first immunocytochemical demonstration of an InsP3 receptor within a cell.

Original languageEnglish
Pages (from-to)468-470
Number of pages3
JournalNature
Volume339
Issue number6224
Publication statusPublished - 1989

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Inositol 1,4,5-Trisphosphate Receptors
Smooth Endoplasmic Reticulum
Rough Endoplasmic Reticulum
Purkinje Cells
Endoplasmic Reticulum
Organelles
Inositol 1,4,5-Trisphosphate
Nuclear Envelope
Neurotransmitter Agents
Intercellular Signaling Peptides and Proteins
Mitochondria
Cell Membrane
Hormones
Electrons
Calcium
Membranes
Antibodies
Population

ASJC Scopus subject areas

  • General

Cite this

Ross, C. A., Meldolesi, J., Milner, T. A., Satoh, T., Supattapone, S., & H. Snyder, S. (1989). Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons. Nature, 339(6224), 468-470.

Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons. / Ross, Christopher A.; Meldolesi, Jacopo; Milner, Teresa A.; Satoh, Tomohide; Supattapone, Surachai; H. Snyder, Solomon.

In: Nature, Vol. 339, No. 6224, 1989, p. 468-470.

Research output: Contribution to journalArticle

Ross, CA, Meldolesi, J, Milner, TA, Satoh, T, Supattapone, S & H. Snyder, S 1989, 'Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons', Nature, vol. 339, no. 6224, pp. 468-470.
Ross CA, Meldolesi J, Milner TA, Satoh T, Supattapone S, H. Snyder S. Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons. Nature. 1989;339(6224):468-470.
Ross, Christopher A. ; Meldolesi, Jacopo ; Milner, Teresa A. ; Satoh, Tomohide ; Supattapone, Surachai ; H. Snyder, Solomon. / Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons. In: Nature. 1989 ; Vol. 339, No. 6224. pp. 468-470.
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