Inositol hexakisphosphate (phytic acid) enhances Ca2+ influx and D-[3H]aspartate release in cultured cerebellar neurons

F. Nicoletti, V. Bruno, L. Fiore, S. Cavallaro, P. L. Canonico

Research output: Contribution to journalArticle

Abstract

Inositol hexakisphosphate (InsP6) increased 45Ca2+ uptake in cultured cerebellar granule cells. This increase was concentration dependent (EC50 = 20 μM), exhibited slow kinetics, and was present after 5 days of cell maturation in culture. InsP6 also enhanced D-[3H]aspartate release in cerebellar granule cells at 11-12 days in vitro. Stimulation of 45Ca2+ uptake was also produced by inositol pentakisphosphate but not by inositol 1,3,4,5-tetrakisphosphate. The increase in 45Ca2+ influx induced by InsP6 was independent of extracellular Na+ and was only partially reduced by the organic calcium blocker nifedipine. The intrinsic action of InsP6 was not affected by competitive or noncompetitive glutamate receptor antagonists. In addition, stimulations of 45Ca2+ uptake by InsP6 and glutamate were additive. These data provide evidence that InsP6 directly activates a specific population of neurons in the CNS.

Original languageEnglish
Pages (from-to)1026-1030
Number of pages5
JournalJournal of Neurochemistry
Volume53
Issue number4
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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