TY - JOUR
T1 - Insertion sites in engrafted cells cluster within a limited repertoire of genomic areas after gammaretroviral vector gene therapy
AU - Deichmann, Annette
AU - Brugman, Martijn H.
AU - Bartholomae, Cynthia C.
AU - Schwarzwaelder, Kerstin
AU - Verstegen, Monique M A
AU - Howe, Steven J.
AU - Arens, Anne
AU - Ott, Marion G.
AU - Hoelzer, Dieter
AU - Seger, Reinhard
AU - Grez, Manuel
AU - Hacein-Bey-Abina, Salima
AU - Cavazzana-Calvo, Marina
AU - Fischer, Alain
AU - Paruzynski, Anna
AU - Gabriel, Richard
AU - Glimm, Hanno
AU - Abel, Ulrich
AU - Cattoglio, Claudia
AU - Mavilio, Fulvio
AU - Cassani, Barbara
AU - Aiuti, Alessandro
AU - Dunbar, Cynthia E.
AU - Baum, Christopher
AU - Gaspar, H. Bobby
AU - Thrasher, Adrian J.
AU - Von Kalle, Christof
AU - Schmidt, Manfred
AU - Wagemaker, Gerard
PY - 2011/11
Y1 - 2011/11
N2 - Vector-associated side effects in clinical gene therapy have provided insights into the molecular mechanisms of hematopoietic regulation in vivo. Surprisingly, many retrovirus insertion sites (RIS) present in engrafted cells have been found to cluster nonrandomly in close association with specific genes. Our data demonstrate that these genes directly influence the in vivo fate of hematopoietic cell clones. Analysis of insertions thus far has been limited to individual clinical studies. Here, we studied 7,000 insertions retrieved from various studies. More than 40% of all insertions found in engrafted gene-modified cells were clustered in the same genomic areas covering only 0.36% of the genome. Gene classification analyses displayed significant overrepresentation of genes associated with hematopoietic functions and relevance for cell growth and survival in vivo. The similarity of insertion distributions indicates that vector insertions in repopulating cells cluster in predictable patterns. Thus, insertion analyses of preclinical in vitro and murine in vivo studies as well as vector insertion repertoires in clinical trials yielded concerted results and mark a small number of interesting genomic loci and genes that warrants further investigation of the biological consequences of vector insertions.
AB - Vector-associated side effects in clinical gene therapy have provided insights into the molecular mechanisms of hematopoietic regulation in vivo. Surprisingly, many retrovirus insertion sites (RIS) present in engrafted cells have been found to cluster nonrandomly in close association with specific genes. Our data demonstrate that these genes directly influence the in vivo fate of hematopoietic cell clones. Analysis of insertions thus far has been limited to individual clinical studies. Here, we studied 7,000 insertions retrieved from various studies. More than 40% of all insertions found in engrafted gene-modified cells were clustered in the same genomic areas covering only 0.36% of the genome. Gene classification analyses displayed significant overrepresentation of genes associated with hematopoietic functions and relevance for cell growth and survival in vivo. The similarity of insertion distributions indicates that vector insertions in repopulating cells cluster in predictable patterns. Thus, insertion analyses of preclinical in vitro and murine in vivo studies as well as vector insertion repertoires in clinical trials yielded concerted results and mark a small number of interesting genomic loci and genes that warrants further investigation of the biological consequences of vector insertions.
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U2 - 10.1038/mt.2011.178
DO - 10.1038/mt.2011.178
M3 - Article
C2 - 21862999
AN - SCOPUS:84860389382
VL - 19
SP - 2031
EP - 2039
JO - Molecular Therapy
JF - Molecular Therapy
SN - 1525-0016
IS - 11
ER -