Insulin and glucagon release of human islets in vitro: Effects of chronic exposure to glucagon

F. Bertuzzi, C. Berra, C. Socci, A. M. Davalli, G. Pozza, A. E. Pontiroli

Research output: Contribution to journalArticle

Abstract

Hyperglucagonemia is commonly found in insulin-dependent as well as in non-insulin-dependent diabetes mellitus, and is likely to be caused by absolute or relative insulin deficiency. The aim of the present study was to evaluate whether a chronic glucagon exposure (1.0 μM for 4 h) modifies the insulin response to acute stimuli with glucagon (1.0 μM), arginine (10.0 mM) and glucose (16.7 mM), or the glucagon response to arginine and glucose, in human islets. Chronic exposure to glucagon did not affect the insulin response to glucose and arginine, but inhibited its response to glucagon (44.6 ± 9.3 vs 168.6 ± 52.3 pg/islet per 20 min, P <0.05); the latter effect was not observed when exposure to glucagon was discontinuous (2.0 μM glucagon alternated with control medium for 30 min periods). The chronic exposure to glucagon also reduced the glucagon response to arginine (-4.9 ± 5.7 vs 19.9 ± 7.9 pg/islet per 20 min, P <0.05) without affecting the inhibition of glucagon release exerted by glucose. These data indicate that chronic exposure to glucagon desensitizes pancreatic α and β cells in response to selected stimuli.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalJournal of Endocrinology
Volume152
Issue number2
Publication statusPublished - Feb 1997

Fingerprint

Glucagon
Insulin
Arginine
Glucose
In Vitro Techniques
Type 2 Diabetes Mellitus

ASJC Scopus subject areas

  • Endocrinology

Cite this

Insulin and glucagon release of human islets in vitro : Effects of chronic exposure to glucagon. / Bertuzzi, F.; Berra, C.; Socci, C.; Davalli, A. M.; Pozza, G.; Pontiroli, A. E.

In: Journal of Endocrinology, Vol. 152, No. 2, 02.1997, p. 239-243.

Research output: Contribution to journalArticle

Bertuzzi, F, Berra, C, Socci, C, Davalli, AM, Pozza, G & Pontiroli, AE 1997, 'Insulin and glucagon release of human islets in vitro: Effects of chronic exposure to glucagon', Journal of Endocrinology, vol. 152, no. 2, pp. 239-243.
Bertuzzi, F. ; Berra, C. ; Socci, C. ; Davalli, A. M. ; Pozza, G. ; Pontiroli, A. E. / Insulin and glucagon release of human islets in vitro : Effects of chronic exposure to glucagon. In: Journal of Endocrinology. 1997 ; Vol. 152, No. 2. pp. 239-243.
@article{be4e7513b4494873951b5bd36ddff607,
title = "Insulin and glucagon release of human islets in vitro: Effects of chronic exposure to glucagon",
abstract = "Hyperglucagonemia is commonly found in insulin-dependent as well as in non-insulin-dependent diabetes mellitus, and is likely to be caused by absolute or relative insulin deficiency. The aim of the present study was to evaluate whether a chronic glucagon exposure (1.0 μM for 4 h) modifies the insulin response to acute stimuli with glucagon (1.0 μM), arginine (10.0 mM) and glucose (16.7 mM), or the glucagon response to arginine and glucose, in human islets. Chronic exposure to glucagon did not affect the insulin response to glucose and arginine, but inhibited its response to glucagon (44.6 ± 9.3 vs 168.6 ± 52.3 pg/islet per 20 min, P <0.05); the latter effect was not observed when exposure to glucagon was discontinuous (2.0 μM glucagon alternated with control medium for 30 min periods). The chronic exposure to glucagon also reduced the glucagon response to arginine (-4.9 ± 5.7 vs 19.9 ± 7.9 pg/islet per 20 min, P <0.05) without affecting the inhibition of glucagon release exerted by glucose. These data indicate that chronic exposure to glucagon desensitizes pancreatic α and β cells in response to selected stimuli.",
author = "F. Bertuzzi and C. Berra and C. Socci and Davalli, {A. M.} and G. Pozza and Pontiroli, {A. E.}",
year = "1997",
month = "2",
language = "English",
volume = "152",
pages = "239--243",
journal = "Journal of Endocrinology",
issn = "0022-0795",
publisher = "BioScientifica Ltd.",
number = "2",

}

TY - JOUR

T1 - Insulin and glucagon release of human islets in vitro

T2 - Effects of chronic exposure to glucagon

AU - Bertuzzi, F.

AU - Berra, C.

AU - Socci, C.

AU - Davalli, A. M.

AU - Pozza, G.

AU - Pontiroli, A. E.

PY - 1997/2

Y1 - 1997/2

N2 - Hyperglucagonemia is commonly found in insulin-dependent as well as in non-insulin-dependent diabetes mellitus, and is likely to be caused by absolute or relative insulin deficiency. The aim of the present study was to evaluate whether a chronic glucagon exposure (1.0 μM for 4 h) modifies the insulin response to acute stimuli with glucagon (1.0 μM), arginine (10.0 mM) and glucose (16.7 mM), or the glucagon response to arginine and glucose, in human islets. Chronic exposure to glucagon did not affect the insulin response to glucose and arginine, but inhibited its response to glucagon (44.6 ± 9.3 vs 168.6 ± 52.3 pg/islet per 20 min, P <0.05); the latter effect was not observed when exposure to glucagon was discontinuous (2.0 μM glucagon alternated with control medium for 30 min periods). The chronic exposure to glucagon also reduced the glucagon response to arginine (-4.9 ± 5.7 vs 19.9 ± 7.9 pg/islet per 20 min, P <0.05) without affecting the inhibition of glucagon release exerted by glucose. These data indicate that chronic exposure to glucagon desensitizes pancreatic α and β cells in response to selected stimuli.

AB - Hyperglucagonemia is commonly found in insulin-dependent as well as in non-insulin-dependent diabetes mellitus, and is likely to be caused by absolute or relative insulin deficiency. The aim of the present study was to evaluate whether a chronic glucagon exposure (1.0 μM for 4 h) modifies the insulin response to acute stimuli with glucagon (1.0 μM), arginine (10.0 mM) and glucose (16.7 mM), or the glucagon response to arginine and glucose, in human islets. Chronic exposure to glucagon did not affect the insulin response to glucose and arginine, but inhibited its response to glucagon (44.6 ± 9.3 vs 168.6 ± 52.3 pg/islet per 20 min, P <0.05); the latter effect was not observed when exposure to glucagon was discontinuous (2.0 μM glucagon alternated with control medium for 30 min periods). The chronic exposure to glucagon also reduced the glucagon response to arginine (-4.9 ± 5.7 vs 19.9 ± 7.9 pg/islet per 20 min, P <0.05) without affecting the inhibition of glucagon release exerted by glucose. These data indicate that chronic exposure to glucagon desensitizes pancreatic α and β cells in response to selected stimuli.

UR - http://www.scopus.com/inward/record.url?scp=0031050159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031050159&partnerID=8YFLogxK

M3 - Article

C2 - 9071981

AN - SCOPUS:0031050159

VL - 152

SP - 239

EP - 243

JO - Journal of Endocrinology

JF - Journal of Endocrinology

SN - 0022-0795

IS - 2

ER -