Insulin and IGF-I phosphorylate eNOS in HUVECs by a caveolin-1 dependent mechanism

Silvia Repetto, Barbara Salani, Davide Maggi, Renzo Cordera

Research output: Contribution to journalArticle

Abstract

Caveolae are plasmamembrane regions which take part in the regulation of intracellular trafficking and signaling of tyrosine kinase receptors. Insulin and IGF-I receptors and their intracellular substrates localize in caveolae. Also eNOS is targeted to caveolae and caveolin-1, the major caveolar protein, acts as a regulator of eNOS activity. Since Insulin and IGF-I phosphorylate and activate eNOS, we investigated the role of caveolin-1 in Insulin and IGF-I stimulated eNOS activity. Here we show that: (1) in human endothelial cells, Insulin and IGF-I stimulate eNOS phosphorylation in a different manner both qualitatively and quantitatively; (2) caveolin-1 down regulation abolishes Insulin and IGF-I stimulated eNOS phosphorylation. These results suggest that caveolae could represent an intracellular site that contributes to differentiate IR and IGF-IR activity, and demonstrate the role of caveolin-1 in the eNOS activation by Insulin and IGF-I.

Original languageEnglish
Pages (from-to)849-852
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number3
DOIs
Publication statusPublished - Nov 25 2005

Keywords

  • Caveolin-1
  • eNOS
  • HUVECs
  • IGF-I receptor
  • Insulin receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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