Insulin and IGF-I phosphorylate eNOS in HUVECs by a caveolin-1 dependent mechanism

Silvia Repetto, Barbara Salani, Davide Maggi, Renzo Cordera

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Caveolae are plasmamembrane regions which take part in the regulation of intracellular trafficking and signaling of tyrosine kinase receptors. Insulin and IGF-I receptors and their intracellular substrates localize in caveolae. Also eNOS is targeted to caveolae and caveolin-1, the major caveolar protein, acts as a regulator of eNOS activity. Since Insulin and IGF-I phosphorylate and activate eNOS, we investigated the role of caveolin-1 in Insulin and IGF-I stimulated eNOS activity. Here we show that: (1) in human endothelial cells, Insulin and IGF-I stimulate eNOS phosphorylation in a different manner both qualitatively and quantitatively; (2) caveolin-1 down regulation abolishes Insulin and IGF-I stimulated eNOS phosphorylation. These results suggest that caveolae could represent an intracellular site that contributes to differentiate IR and IGF-IR activity, and demonstrate the role of caveolin-1 in the eNOS activation by Insulin and IGF-I.

Original languageEnglish
Pages (from-to)849-852
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number3
DOIs
Publication statusPublished - Nov 25 2005

Fingerprint

Caveolin 1
Insulin-Like Growth Factor I
Caveolae
Insulin
Phosphorylation
IGF Type 1 Receptor
Endothelial cells
Receptor Protein-Tyrosine Kinases
Down-Regulation
Endothelial Cells
Chemical activation
Substrates
Proteins

Keywords

  • Caveolin-1
  • eNOS
  • HUVECs
  • IGF-I receptor
  • Insulin receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Insulin and IGF-I phosphorylate eNOS in HUVECs by a caveolin-1 dependent mechanism. / Repetto, Silvia; Salani, Barbara; Maggi, Davide; Cordera, Renzo.

In: Biochemical and Biophysical Research Communications, Vol. 337, No. 3, 25.11.2005, p. 849-852.

Research output: Contribution to journalArticle

Repetto, Silvia ; Salani, Barbara ; Maggi, Davide ; Cordera, Renzo. / Insulin and IGF-I phosphorylate eNOS in HUVECs by a caveolin-1 dependent mechanism. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 337, No. 3. pp. 849-852.
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