TY - JOUR
T1 - Insulin and IGFs induce apoptosis in chick embryo retinas deprived of L-glutamine
AU - Calvaruso, Giuseppe
AU - Vento, Renza
AU - Gerbino, Elvira
AU - Lauricella, Marianna
AU - Carabillò, Maria
AU - Main, Helen
AU - Tesoriere, Giovanni
PY - 1997/4
Y1 - 1997/4
N2 - In chick embryo retinas, cultured in serum-free medium lacking L-glutamine, IGF-I, IGF-II and insulin induced apoptotic DNA fragmentation and cell death, IGF-I being the most efficacious compound. The apoptotic effect, which was particularly evident in retinas removed from 7-day-old chick embryos, declined with the age of the embryos and disappeared after day 11. Apoptosis appeared after a time lag of 8 h and then increased with time up to 16 h. Cycloheximide, an inhibitor of protein synthesis, was capable of entirely abolishing apoptotic cell death. The effect induced by IGFs or insulin was suppressed by the addition of glutamine. Cytokine-mediated apoptosis was also observed after withdrawal of phosphate. We suggest that IGFs or insulin may produce, in retinas cultured in medium lacking L-glutamine or phosphate, a conflict of signals that could be lethal for retinal cells.
AB - In chick embryo retinas, cultured in serum-free medium lacking L-glutamine, IGF-I, IGF-II and insulin induced apoptotic DNA fragmentation and cell death, IGF-I being the most efficacious compound. The apoptotic effect, which was particularly evident in retinas removed from 7-day-old chick embryos, declined with the age of the embryos and disappeared after day 11. Apoptosis appeared after a time lag of 8 h and then increased with time up to 16 h. Cycloheximide, an inhibitor of protein synthesis, was capable of entirely abolishing apoptotic cell death. The effect induced by IGFs or insulin was suppressed by the addition of glutamine. Cytokine-mediated apoptosis was also observed after withdrawal of phosphate. We suggest that IGFs or insulin may produce, in retinas cultured in medium lacking L-glutamine or phosphate, a conflict of signals that could be lethal for retinal cells.
KW - Apoptosis
KW - Chick embryo retina
KW - Development
KW - Growth factors
UR - http://www.scopus.com/inward/record.url?scp=0030978032&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030978032&partnerID=8YFLogxK
M3 - Article
C2 - 16465230
AN - SCOPUS:0030978032
VL - 4
SP - 209
EP - 215
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
SN - 1350-9047
IS - 3
ER -