TY - JOUR
T1 - Insulin and intracellular calcium responsiveness to glucagon-like peptide-1 and pituitary adenylate cyclase-activating peptide by dispersed adult porcine islet cells
AU - Davalli, Alberto M.
AU - Bertuzzi, Federico
AU - Meoni, Cesare
AU - Scaglia, Luisa
AU - Socci, Carlo
AU - Pozza, Guido
AU - Pontiroli, Antonio E.
PY - 1999/1/15
Y1 - 1999/1/15
N2 - Background. There is a great need to learn more about porcine islet physiology because porcine islets represent a promising source of xenogeneic tissue for β-cell replacement therapy in humans. Methods. We evaluated the effects of two important physiological regulators of insulin secretion, glucagon-like peptide-1 (GLP-1) and pituitary adenylate cyclase-activating peptide (PACAP), on insulin release and intracellular calcium ([Ca++](i)) by adult porcine islet cells. Results. Exposure to GLP-1 and PACAP significantly potentiated glucose-induced insulin release and improved the sensitivity to glucose as a secretagogue. About 70% of cells stimulated with 20 mmol/L glucose alone showed an increase in [Ca++](i), whereas the addition of GLP-1 and PACAP induced [Ca++](i) increases in 86% and 93% of cells, respectively. Conclusions. The good insulin and [Ca++](i) responsiveness of porcine islet cells to both GLP-1 and PACAP provides an additional proof of their suitability for transplantation.
AB - Background. There is a great need to learn more about porcine islet physiology because porcine islets represent a promising source of xenogeneic tissue for β-cell replacement therapy in humans. Methods. We evaluated the effects of two important physiological regulators of insulin secretion, glucagon-like peptide-1 (GLP-1) and pituitary adenylate cyclase-activating peptide (PACAP), on insulin release and intracellular calcium ([Ca++](i)) by adult porcine islet cells. Results. Exposure to GLP-1 and PACAP significantly potentiated glucose-induced insulin release and improved the sensitivity to glucose as a secretagogue. About 70% of cells stimulated with 20 mmol/L glucose alone showed an increase in [Ca++](i), whereas the addition of GLP-1 and PACAP induced [Ca++](i) increases in 86% and 93% of cells, respectively. Conclusions. The good insulin and [Ca++](i) responsiveness of porcine islet cells to both GLP-1 and PACAP provides an additional proof of their suitability for transplantation.
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U2 - 10.1097/00007890-199901150-00028
DO - 10.1097/00007890-199901150-00028
M3 - Article
C2 - 9921815
AN - SCOPUS:0033556562
VL - 67
SP - 174
EP - 176
JO - Transplantation
JF - Transplantation
SN - 0041-1337
IS - 1
ER -