Insulin-like growth factor 1 receptor signaling induced by supraphysiological doses of IGF-1 in human peripheral blood lymphocytes

Sara Spaziani, Esther Imperlini, Annamaria Mancini, Marianna Caterino, Pasqualina Buono, Stefania Orrù

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Insulin-like growth factor-1 (IGF-1) mediates some of growth hormone anabolic functions through its receptor, IGF-1R. Following ligand binding, intracellular signaling pathways are activated favouring proliferation, cell survival, tissue growth, development, and differentiation. IGF-1 is included in the World Anti-Doping Agency Prohibited List. While the evidence for IGF-1 as performance-enhancing substrate in healthy humans is still weak, clinical studies demonstrated that the endogenous growth hormone/IGF-1 excess is associated with cardiovascular implications. Previously, we demonstrated that human peripheral blood lymphocytes represent a suitable system to identify a gene signature, related to dihydrotestosterone or IGF-1 abuse, independent from the type of sport. In addition, in a proteomic study, we demonstrated that dihydrotestosterone hyperdosage affects cell motility and apoptosis. Here, we investigate the doping action of IGF-1 by means of a differential proteomic approach and specific protein arrays, revealing an active cytoskeletal reorganization mediated by Stat-1; moreover, IGF-1 stimulation produces a sustained activation of different signaling pathways as well as an overproduction of cytokines positively related to immune response and inflammation. In conclusion, these data indicate that, following IGF-1 hyperdosage, circulating peripheral blood lymphocytes could be more prone to transendothelial migration.

Original languageEnglish
Pages (from-to)1623-1629
Number of pages7
JournalProteomics
Volume14
Issue number13-14
DOIs
Publication statusPublished - 2014

Fingerprint

Somatomedin Receptors
Lymphocytes
Somatomedins
Blood
Dihydrotestosterone
Proteomics
Growth Hormone
Doping (additives)
Transendothelial and Transepithelial Migration
Protein Array Analysis
Cell proliferation
Sports
Growth and Development
Cell Movement
Cell Survival
Genes
Chemical activation
Tissue
Apoptosis
Cytokines

Keywords

  • 2D DIGE
  • Cell biology
  • IGF-1
  • IGF-1 receptor
  • Lymphocytes
  • Peptide hormone

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Medicine(all)

Cite this

Insulin-like growth factor 1 receptor signaling induced by supraphysiological doses of IGF-1 in human peripheral blood lymphocytes. / Spaziani, Sara; Imperlini, Esther; Mancini, Annamaria; Caterino, Marianna; Buono, Pasqualina; Orrù, Stefania.

In: Proteomics, Vol. 14, No. 13-14, 2014, p. 1623-1629.

Research output: Contribution to journalArticle

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