Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats

Nicola Basso, Emanuele Soricelli, Lidia Castagneto-Gissey, Giovanni Casella, Davide Albanese, Francesca Fava, Claudio Donati, Kieran Tuohy, Giulia Angelini, Federica La Neve, Anna Severino, Virginia Kamvissi-Lorenz, Andrea L. Birkenfeld, Stefan Bornstein, Melania Manco, Geltrude Mingrone

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Metabolic surgery improves insulin resistance and type 2 diabetes possibly because of weight loss. We performed a novel sleeve gastrectomy in rats that resects ∼80% of the glandular portion, leaving the forestomach almost intact (glandular gastrectomy [GG]) and compared subsequent metabolic remodeling with a sham operation. GG did not affect body weight, at least after 10 weeks; improved hepatic and peripheral insulin sensitivity likely through increased Akt, glycogen synthase kinase 3, and AMPK phosphorylation; and reduced ectopic fat deposition and hepatic glycogen overaccumulation. Body adipose tissue was redistributed, with reduction of intraabdominal fat. We found a reduction of circulating ghrelin levels, increased GLP-1 plasma concentration, and remodeling of gut microbiome diversity characterized by a lower relative abundance of Ruminococcus and a higher relative abundance of Lactobacillus and Collinsella. These data suggest that at least in rat, the glandular stomach plays a central role in the improvement of insulin resistance, even if obesity persists. GG provides a new model of the metabolically healthy obese phenotype.

Original languageEnglish
Pages (from-to)2990-3001
Number of pages12
JournalDiabetes
Volume65
Issue number10
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Microbiota
Gastrectomy
Insulin Resistance
Fats
Ruminococcus
Glycogen Synthase Kinase 3
Liver Glycogen
AMP-Activated Protein Kinases
Ghrelin
Glucagon-Like Peptide 1
Bariatric Surgery
Lactobacillus
Type 2 Diabetes Mellitus
Adipose Tissue
Weight Loss
Stomach
Obesity
Body Weight
Phosphorylation
Phenotype

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Basso, N., Soricelli, E., Castagneto-Gissey, L., Casella, G., Albanese, D., Fava, F., ... Mingrone, G. (2016). Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats. Diabetes, 65(10), 2990-3001. https://doi.org/10.2337/db16-0039

Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats. / Basso, Nicola; Soricelli, Emanuele; Castagneto-Gissey, Lidia; Casella, Giovanni; Albanese, Davide; Fava, Francesca; Donati, Claudio; Tuohy, Kieran; Angelini, Giulia; Neve, Federica La; Severino, Anna; Kamvissi-Lorenz, Virginia; Birkenfeld, Andrea L.; Bornstein, Stefan; Manco, Melania; Mingrone, Geltrude.

In: Diabetes, Vol. 65, No. 10, 01.10.2016, p. 2990-3001.

Research output: Contribution to journalArticle

Basso, N, Soricelli, E, Castagneto-Gissey, L, Casella, G, Albanese, D, Fava, F, Donati, C, Tuohy, K, Angelini, G, Neve, FL, Severino, A, Kamvissi-Lorenz, V, Birkenfeld, AL, Bornstein, S, Manco, M & Mingrone, G 2016, 'Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats', Diabetes, vol. 65, no. 10, pp. 2990-3001. https://doi.org/10.2337/db16-0039
Basso N, Soricelli E, Castagneto-Gissey L, Casella G, Albanese D, Fava F et al. Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats. Diabetes. 2016 Oct 1;65(10):2990-3001. https://doi.org/10.2337/db16-0039
Basso, Nicola ; Soricelli, Emanuele ; Castagneto-Gissey, Lidia ; Casella, Giovanni ; Albanese, Davide ; Fava, Francesca ; Donati, Claudio ; Tuohy, Kieran ; Angelini, Giulia ; Neve, Federica La ; Severino, Anna ; Kamvissi-Lorenz, Virginia ; Birkenfeld, Andrea L. ; Bornstein, Stefan ; Manco, Melania ; Mingrone, Geltrude. / Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats. In: Diabetes. 2016 ; Vol. 65, No. 10. pp. 2990-3001.
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