Intact human lymphocyte membranes respond to muscarinic receptor stimulation by oxotremorine with marked changes in microviscosity and an increase in cyclic GMP

P. Masturzo, M. Salmona, O. Nordstrom, S. Consolo, H. Ladinsky

Research output: Contribution to journalArticlepeer-review

Abstract

The muscarinic agonist oxotremorine produced a linear dose-dependent increase in membrane fluidity of intact and viable human lymphocytes in vitro. This effect proved to be receptor-mediated because preincubation with 10-5 M atropine shifted the dose-response curve one order of magnitude rightward. Pirenzepine preincubation did not affect membrane fluidity variation. A cGMP increase was also found after oxotremorine treatment. The results are discussed in terms of possible modulation ofguanyl cyclase and adenyl cyclase through membrane fluidity variations.

Original languageEnglish
Pages (from-to)194-198
Number of pages5
JournalFEBS Letters
Volume192
Issue number2
DOIs
Publication statusPublished - Nov 18 1985

Keywords

  • Atropine
  • Cyclic GMP
  • Lymphocyte
  • Membrane fluidity
  • Oxotremorine
  • Pirenzepine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Intact human lymphocyte membranes respond to muscarinic receptor stimulation by oxotremorine with marked changes in microviscosity and an increase in cyclic GMP'. Together they form a unique fingerprint.

Cite this