Intact human lymphocyte membranes respond to muscarinic receptor stimulation by oxotremorine with marked changes in microviscosity and an increase in cyclic GMP

P. Masturzo; M. Salmona; O. Nordstrom; S. Consolo; H. Ladinsky

doi:10.1016/0014-5793(85)80106-X

Intact human lymphocyte membranes respond to muscarinic receptor stimulation by oxotremorine with marked changes in microviscosity and an increase in cyclic GMP

P. Masturzo, M. Salmona, O. Nordstrom, S. Consolo, H. Ladinsky

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article › peer-review

Abstract

The muscarinic agonist oxotremorine produced a linear dose-dependent increase in membrane fluidity of intact and viable human lymphocytes in vitro. This effect proved to be receptor-mediated because preincubation with 10^-5 M atropine shifted the dose-response curve one order of magnitude rightward. Pirenzepine preincubation did not affect membrane fluidity variation. A cGMP increase was also found after oxotremorine treatment. The results are discussed in terms of possible modulation ofguanyl cyclase and adenyl cyclase through membrane fluidity variations.

Original language	English
Pages (from-to)	194-198
Number of pages	5
Journal	FEBS Letters
Volume	192
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)80106-X
Publication status	Published - Nov 18 1985

Keywords

Atropine
Cyclic GMP
Lymphocyte
Membrane fluidity
Oxotremorine
Pirenzepine

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(85)80106-X

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title = "Intact human lymphocyte membranes respond to muscarinic receptor stimulation by oxotremorine with marked changes in microviscosity and an increase in cyclic GMP",

abstract = "The muscarinic agonist oxotremorine produced a linear dose-dependent increase in membrane fluidity of intact and viable human lymphocytes in vitro. This effect proved to be receptor-mediated because preincubation with 10-5 M atropine shifted the dose-response curve one order of magnitude rightward. Pirenzepine preincubation did not affect membrane fluidity variation. A cGMP increase was also found after oxotremorine treatment. The results are discussed in terms of possible modulation ofguanyl cyclase and adenyl cyclase through membrane fluidity variations.",

keywords = "Atropine, Cyclic GMP, Lymphocyte, Membrane fluidity, Oxotremorine, Pirenzepine",

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T1 - Intact human lymphocyte membranes respond to muscarinic receptor stimulation by oxotremorine with marked changes in microviscosity and an increase in cyclic GMP

AU - Masturzo, P.

AU - Salmona, M.

AU - Nordstrom, O.

AU - Consolo, S.

AU - Ladinsky, H.

PY - 1985/11/18

Y1 - 1985/11/18

N2 - The muscarinic agonist oxotremorine produced a linear dose-dependent increase in membrane fluidity of intact and viable human lymphocytes in vitro. This effect proved to be receptor-mediated because preincubation with 10-5 M atropine shifted the dose-response curve one order of magnitude rightward. Pirenzepine preincubation did not affect membrane fluidity variation. A cGMP increase was also found after oxotremorine treatment. The results are discussed in terms of possible modulation ofguanyl cyclase and adenyl cyclase through membrane fluidity variations.

AB - The muscarinic agonist oxotremorine produced a linear dose-dependent increase in membrane fluidity of intact and viable human lymphocytes in vitro. This effect proved to be receptor-mediated because preincubation with 10-5 M atropine shifted the dose-response curve one order of magnitude rightward. Pirenzepine preincubation did not affect membrane fluidity variation. A cGMP increase was also found after oxotremorine treatment. The results are discussed in terms of possible modulation ofguanyl cyclase and adenyl cyclase through membrane fluidity variations.

KW - Atropine

KW - Cyclic GMP

KW - Lymphocyte

KW - Membrane fluidity

KW - Oxotremorine

KW - Pirenzepine

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Intact human lymphocyte membranes respond to muscarinic receptor stimulation by oxotremorine with marked changes in microviscosity and an increase in cyclic GMP

Abstract

Keywords

ASJC Scopus subject areas

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