Integrating miRNA and gene expression profiling analysis revealed regulatory networks in gastrointestinal stromal tumors

Maria Abbondanza Pantaleo, Gloria Ravegnini, Annalisa Astolfi, Vittorio Simeon, Margherita Nannini, Maristella Saponara, Milena Urbini, Lidia Gatto, Valentina Indio, Giulia Sammarini, Donatella Santini, Manuela Ferracin, Massimo Negrini, Patrizia Hrelia, Guido Biasco, Sabrina Angelini

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: Currently, little is known about differences in miRNA expression between KIT/PDGFRA mutant and KIT/PDGFRA wild-type (WT)-SDH deficient gastrointestinal stromal tumors (GIST). This prompted us to perform an integrated multiple expression profile of miRNA and mRNA, constructing an original miRNA-mRNA regulatory network in KIT/PDGFRA WT-SDH deficient GIST patients. Patients & methods: Analyses were carried out on KIT/PDGFRA mutant versus KIT/PDGFRA WT-SDH deficient GIST. Genome-wide miRNA and gene-expression analysis were performed using Agilent Human miRNA microarray and Affimetrix array, respectively. Results: Three potential regulatory networks (IGF1R → miR-139-5p/miR-455/let-7b, cyclin-dependent kinase 6 (CDK6) → miR-139-5p/let-7b and CD44 → miR-330-3p) were identified. Conclusion: The miR-139-5p, 455-5p and let-7b signature, in particular, may represent an important therapeutic target in KIT/PDGFRA WT-SDH deficient GIST, usually characterized by IGF1R overexpression.

Original languageEnglish
Pages (from-to)1347-1366
Number of pages20
JournalEpigenomics
Volume8
Issue number10
DOIs
Publication statusPublished - Oct 1 2016

Keywords

  • GIST
  • IGF1R
  • KIT
  • let-7b
  • miR-455-5p
  • miR139-5p
  • PDGFRA
  • WT-SDH deficient

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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