Abstract
We have studied the genetic profile of the host genome and hepatitis B virus (HBV) in HBV-associated primary hepatocellular carcinoma (HCC). Comparative analyses of HCC cell line Hep 40 and the original biopsy specimens showed the episomal and replicating form of HBV only in the biopsy specimen from nontumor (NT) cirrhotic liver tissue, where a molecular change in the 1p36 region was detected (NT tissue showed a normal 46XY karyotype). In contrast, only integrated HBV was detected in HCC tumor (T) tissue and Hep 40 cells. Two HBV integration sites were identical in HCC tissue and the hyperdiploid Hep 40 cell line, where genetic alteration in the 1p36 region was identified. These data indicate that viral replication is ongoing only in NT cirrhotic-hyperplastic, chromosomally normal tissue with evidence for genetic instability. Only the tumor cell with altered genotype has virus integrated.
| Original language | English |
|---|---|
| Pages (from-to) | 1393-1398 |
| Number of pages | 6 |
| Journal | Hepatology |
| Volume | 22 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1995 |
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ASJC Scopus subject areas
- Hepatology
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Integration of hepatitis B virus and alteration of the 1p36 Region found in cancerous tissue of primary hepatocellular carcinoma with viral replication evidenced only in noncancerous, cirrhotic tissue. / Simon, Daniela; Carr, Brian I.
In: Hepatology, Vol. 22, No. 5, 1995, p. 1393-1398.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Integration of hepatitis B virus and alteration of the 1p36 Region found in cancerous tissue of primary hepatocellular carcinoma with viral replication evidenced only in noncancerous, cirrhotic tissue
AU - Simon, Daniela
AU - Carr, Brian I.
PY - 1995
Y1 - 1995
N2 - We have studied the genetic profile of the host genome and hepatitis B virus (HBV) in HBV-associated primary hepatocellular carcinoma (HCC). Comparative analyses of HCC cell line Hep 40 and the original biopsy specimens showed the episomal and replicating form of HBV only in the biopsy specimen from nontumor (NT) cirrhotic liver tissue, where a molecular change in the 1p36 region was detected (NT tissue showed a normal 46XY karyotype). In contrast, only integrated HBV was detected in HCC tumor (T) tissue and Hep 40 cells. Two HBV integration sites were identical in HCC tissue and the hyperdiploid Hep 40 cell line, where genetic alteration in the 1p36 region was identified. These data indicate that viral replication is ongoing only in NT cirrhotic-hyperplastic, chromosomally normal tissue with evidence for genetic instability. Only the tumor cell with altered genotype has virus integrated.
AB - We have studied the genetic profile of the host genome and hepatitis B virus (HBV) in HBV-associated primary hepatocellular carcinoma (HCC). Comparative analyses of HCC cell line Hep 40 and the original biopsy specimens showed the episomal and replicating form of HBV only in the biopsy specimen from nontumor (NT) cirrhotic liver tissue, where a molecular change in the 1p36 region was detected (NT tissue showed a normal 46XY karyotype). In contrast, only integrated HBV was detected in HCC tumor (T) tissue and Hep 40 cells. Two HBV integration sites were identical in HCC tissue and the hyperdiploid Hep 40 cell line, where genetic alteration in the 1p36 region was identified. These data indicate that viral replication is ongoing only in NT cirrhotic-hyperplastic, chromosomally normal tissue with evidence for genetic instability. Only the tumor cell with altered genotype has virus integrated.
UR - http://www.scopus.com/inward/record.url?scp=0028789049&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028789049&partnerID=8YFLogxK
U2 - 10.1016/0270-9139(95)90142-6
DO - 10.1016/0270-9139(95)90142-6
M3 - Article
C2 - 7590653
AN - SCOPUS:0028789049
VL - 22
SP - 1393
EP - 1398
JO - Hepatology
JF - Hepatology
SN - 0270-9139
IS - 5
ER -