Integration of multiple platforms for the analysis of multifluorescent marking technology applied to pediatric GBM and dipg

Giulia Pericoli, Stefania Petrini, Ezio Giorda, Roberta Ferretti, Maria Antonietta Ajmone-Cat, Will Court, Libenzio Adrian Conti, Roberta De Simone, Paola Bencivenga, Alessia Palma, Angela Di Giannatale, Chris Jones, Andrea Carai, Angela Mastronuzzi, Emmanuel de Billy, Franco Locatelli, Maria Vinci

Research output: Contribution to journalArticlepeer-review


The intratumor heterogeneity represents one of the most difficult challenges for the development of effective therapies to treat pediatric glioblastoma (pGBM) and diffuse intrinsic pontine glioma (DIPG). These brain tumors are composed of heterogeneous cell subpopulations that coexist and cooperate to build a functional network responsible for their aggressive phenotype. Understanding the cellular and molecular mechanisms sustaining such network will be crucial for the identification of new therapeutic strategies. To study more in-depth these mechanisms, we sought to apply the Multifluorescent Marking Technology. We generated multifluorescent pGBM and DIPG bulk cell lines randomly expressing six different fluorescent proteins and from which we derived stable optical barcoded single cell-derived clones. In this study, we focused on the application of the Multifluorescent Marking Technology in 2D and 3D in vitro/ex vivo culture systems. We discuss how we integrated different multimodal fluorescence analysis platforms, identifying their strengths and limitations, to establish the tools that will enable further studies on the intratumor heterogeneity and interclonal interactions in pGBM and DIPG.

Original languageEnglish
Article number6763
Pages (from-to)1-25
Number of pages25
JournalInternational Journal of Molecular Sciences
Issue number18
Publication statusPublished - Sep 2 2020


  • DIPG
  • Fluorescence imaging
  • Heterogeneity
  • Multifluorescent marking technology
  • Optical barcode
  • Pediatric GBM
  • RGB marking

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


Dive into the research topics of 'Integration of multiple platforms for the analysis of multifluorescent marking technology applied to pediatric GBM and dipg'. Together they form a unique fingerprint.

Cite this