Integrative histologic and bioinformatics analysis of BIRC5/survivin expression in oral squamous cell carcinoma

Giuseppe Troiano, Agostino Guida, Gabriella Aquino, Gerardo Botti, Nunzia Simona Losito, Silvana Papagerakis, Maria Carmela Pedicillo, Franco Ionna, Francesco Longo, Monica Cantile, Antonio Pennella, Lucio Lo Russo, Giovanni Di Gioia, Maria Addolorata Mariggiò, Lorenzo Lo Muzio, Giuseppe Pannone

Research output: Contribution to journalArticle

Abstract

Survivin is a well-known protein involved in the inhibition of apoptosis in many different cancer types. The aim of this study was to perform an integrated bioinformatic and histologic analysis in order to study the expression and prognostic role of Survivin and its related gene BIRC5 in oral cancer. Publicly available databases were accessed via Gene Expression Omnibus and Oncomine, in addition raw data from The Cancer Genome Atlas (TCGA) were also obtained in order to analyze the rate of gene mutation, expression and methylation in patients with oral squamous cells carcinoma (OSCC). Immunohistochemistry (IHC) was also performed in order to evaluate the nuclear and cytoplasmic expression of Survivin and their correlation with cell proliferation in samples from OSCC patients. Results of this study revealed that Survivin is rarely mutated in OSCC samples and upregulated when compared to non-cancerous tissue. A negative correlation between the methylation of the island cg25986496 and BIRC5 mRNA expression was detected from TCGA data. IHC staining revealed that cytoplasmic (and not nuclear) expression of Survivin is associated with poor overall survival in OSCC patients, while the nuclear expression correlates with higher proliferation rate. In addition, data from TCGA database revealed that BIRC5 gene expression is an independent prognostic factor for OSCC patients.

Original languageEnglish
Article number2664
JournalInternational Journal of Molecular Sciences
Volume19
Issue number9
DOIs
Publication statusPublished - Sep 8 2018

Keywords

  • Bioinformatic
  • BIRC5
  • Immunohistochemistry
  • Oral cancer
  • Survivin
  • TCGA

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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  • Cite this

    Troiano, G., Guida, A., Aquino, G., Botti, G., Losito, N. S., Papagerakis, S., Pedicillo, M. C., Ionna, F., Longo, F., Cantile, M., Pennella, A., Lo Russo, L., Di Gioia, G., Mariggiò, M. A., Lo Muzio, L., & Pannone, G. (2018). Integrative histologic and bioinformatics analysis of BIRC5/survivin expression in oral squamous cell carcinoma. International Journal of Molecular Sciences, 19(9), [2664]. https://doi.org/10.3390/ijms19092664