Integrin receptor α6β1 is localized at specific sites of cell-to-cell contact in rat seminiferous epithelium

M. Salanova, M. Stefanini, I. De Curtis, F. Palombi

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Abstract

With the aim of investigating the presence and role of integrin receptors in cell-to-cell interactions during spermatogenesis, we have immunolocalized α6A integrin chain in the rat seminiferous epithelium. In both prepubertal and adult seminiferous epithelium, the antigen was found to be restricted to definite sites of intercellular contact, following a stage-specific distribution almost invariably identical to that known for β1 chain. In the adult seminiferous epithelium, positivity for both antigens was found exclusively around the profiles of elongating and maturing spermatids and, in most but not all stages, at a characteristic suprabasal position. In the prepubertal rat, the antigen is localized along a very regular suprabasal line of intercellular contacts. In immunoprecipitation experiments on both seminiferous epithelium explants and Sertoli cell cultures from 3-wk-old rats, anti-α6A antibody coprecipitates a polypeptide of 118 kDa, presumably corresponding to β1 chain. These data strongly suggest that the integrin heterodimer α6Aβ1 is expressed at sites of intercellular contact in the rat seminiferous epithelium. The stage-specific and restricted pattern observed by immunofluorescence suggests that this integrin is involved in regulatory interactions between Sertoli cells and germ cells during spermatogenesis.

Original languageEnglish
Pages (from-to)79-87
Number of pages9
JournalBiology of Reproduction
Volume52
Issue number1
DOIs
Publication statusPublished - 1995

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ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

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