Integrins, muscle agrin and sarcoglycans during muscular inactivity conditions

An immunohistochemical study

G. Anastasi, G. Cutroneo, G. Santoro, A. Arco, G. Rizzo, C. Trommino, P. Bramanti, L. Soscia, Angelo Favaloro

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Sarcoglycans are transmembrane proteins that seem to be functionally and pathologically as important as dystrophin. Sarcoglycans cluster together to form a complex, which is localized in the cell membrane of skeletal, cardiac, and smooth muscle. It has been proposed that the dystrophin-glycoprotein complex (DGC) links the actin cytoskeleton with the extracellular matrix and the proper maintenance of this connection is thought to be crucial to the mechanical stability of the sarcolemma. The integrins are a family of heterodimeric cell surface receptors which play a crucial role in cell adhesion including cell-matrix and intracellular interactions and therefore are involved in various biological phenomena, including cell migration, and differentiation tissue repair. Sarcoglycans and integrins play a mechanical and signaling role stabilizing the systems during cycles of contraction and relaxation. Several studies suggested the possibility that integrins might play a role in muscle agrin signalling. On these basis, we performed an immunohistochemical analyzing sarcoglycans, integrins and agrin, on human skeletal muscle affected by sensitive-motor polyneuropathy, in order to better define the correlation between these proteins and neurogenic atrophy due to peripheral neuropathy. Our results showed the existence of a cascade mechanism which provoke a loss of regulatory effects of muscle activity on costameres, due to loss of muscle and neural agrin. This cascade mechanism could determine a quantitative modification of transmembrane receptors and loss of α7B could be replaced and reinforced by enhanced expression of the α7A integrin to restore muscle fiber viability. Second, it is possible that the reduced cycles of contraction and relaxation of muscle fibers, during muscular atrophy, provoke a loss of mechanical stresses transmitted over cell surface receptors that physically couple the cytoskeleton to extracellular matrix. Consequently, these mechanical changes could determine modifications of chemical signals through variations of pathway structural integrins, and α7A could replace α7B.

Original languageEnglish
Pages (from-to)327-336
Number of pages10
JournalEuropean journal of histochemistry : EJH
Volume50
Issue number4
Publication statusPublished - Oct 2006

Fingerprint

Agrin
Sarcoglycans
integrins
Integrins
Muscles
muscles
dystrophin
Dystrophin
Cell Surface Receptors
cell adhesion
extracellular matrix
muscle fibers
receptors
Extracellular Matrix
skeletal muscle
Costameres
Skeletal Muscle
Biological Phenomena
Sarcolemma
muscular atrophy

Keywords

  • Integrin
  • Muscle agrin
  • Neural agrin
  • Sarcoglycan
  • Skeletal muscle

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Animal Science and Zoology
  • Developmental Biology

Cite this

Anastasi, G., Cutroneo, G., Santoro, G., Arco, A., Rizzo, G., Trommino, C., ... Favaloro, A. (2006). Integrins, muscle agrin and sarcoglycans during muscular inactivity conditions: An immunohistochemical study. European journal of histochemistry : EJH, 50(4), 327-336.

Integrins, muscle agrin and sarcoglycans during muscular inactivity conditions : An immunohistochemical study. / Anastasi, G.; Cutroneo, G.; Santoro, G.; Arco, A.; Rizzo, G.; Trommino, C.; Bramanti, P.; Soscia, L.; Favaloro, Angelo.

In: European journal of histochemistry : EJH, Vol. 50, No. 4, 10.2006, p. 327-336.

Research output: Contribution to journalArticle

Anastasi, G, Cutroneo, G, Santoro, G, Arco, A, Rizzo, G, Trommino, C, Bramanti, P, Soscia, L & Favaloro, A 2006, 'Integrins, muscle agrin and sarcoglycans during muscular inactivity conditions: An immunohistochemical study', European journal of histochemistry : EJH, vol. 50, no. 4, pp. 327-336.
Anastasi, G. ; Cutroneo, G. ; Santoro, G. ; Arco, A. ; Rizzo, G. ; Trommino, C. ; Bramanti, P. ; Soscia, L. ; Favaloro, Angelo. / Integrins, muscle agrin and sarcoglycans during muscular inactivity conditions : An immunohistochemical study. In: European journal of histochemistry : EJH. 2006 ; Vol. 50, No. 4. pp. 327-336.
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