Inter-laboratory consistency and variability in the buccal micronucleus cytome assay depends on biomarker scored and laboratory experience: results from the HUMNxl international inter-laboratory scoring exercise

Claudia Bolognesi, Siegfried Knasmueller, Armen Nersesyan, Paola Roggieri, Marcello Ceppi, Marco Bruzzone, Ewa Blaszczyk, Danuta Mielzynska-Svach, Mirta Milic, Stefano Bonassi, Danieli Benedetti, Juliana da Silva, Raphael Toledo, Daisy Maria Fávero Salvadori, Helena Groot de Restrepo, Metka Filipic, Klara Hercog, Ayça Aktaş, Sema Burgaz, Michael KundiTamara Grummt, Philip Thomas, Maryam Hor, Maria Escudero-Fung, Nina Holland, Michael Fenech

Research output: Contribution to journalArticle

Abstract

The buccal micronucleus cytome (BMNcyt) assay in uncultured exfoliated epithelial cells from oral mucosa is widely applied in biomonitoring human exposures to genotoxic agents and is also proposed as a suitable test for prescreening and follow-up of precancerous oral lesions. The main limitation of the assay is the large variability observed in the baseline values of micronuclei (MNi) and other nuclear anomalies mainly related to different scoring criteria. The aim of this international collaborative study, involving laboratories with different level of experience, was to evaluate the inter- and intra-laboratory variations in the BMNcyt parameters, using recently implemented guidelines, in scoring cells from the same pooled samples obtained from healthy subjects (control group) and from cancer patients undergoing radiotherapy (treated group). The results indicate that all laboratories correctly discriminated samples from the two groups by a significant increase of micronucleus (MN) and nuclear bud (NBUD) frequencies and differentiated binucleated (BN) cells, associated with the exposure to ionizing radiation. The experience of the laboratories was shown to play an important role in the identification of the different cell types and nuclear anomalies. MN frequency in differentiated mononucleated (MONO) and BN cells showed the greatest consistency among the laboratories and low variability was also detected in the frequencies of MONO and BN cells. A larger variability was observed in classifying the different cell types, indicating the subjectivity in the interpretation of some of the scoring criteria while reproducibility of the results between scoring sessions was very good. An inter-laboratory calibration exercise is strongly recommended before starting studies with BMNcyt assay involving multiple research centers.

Original languageEnglish
JournalMutagenesis
DOIs
Publication statusE-pub ahead of print - Sep 26 2016

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Micronucleus Tests
Cheek
Biomarkers
Assays
Exercise
Environmental Monitoring
Ionizing radiation
Radiotherapy
Mouth Mucosa
Ionizing Radiation
Reproducibility of Results
Calibration
Healthy Volunteers
Epithelial Cells
Guidelines
Control Groups
Research

Keywords

  • Journal Article

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Inter-laboratory consistency and variability in the buccal micronucleus cytome assay depends on biomarker scored and laboratory experience : results from the HUMNxl international inter-laboratory scoring exercise. / Bolognesi, Claudia; Knasmueller, Siegfried; Nersesyan, Armen; Roggieri, Paola; Ceppi, Marcello; Bruzzone, Marco; Blaszczyk, Ewa; Mielzynska-Svach, Danuta; Milic, Mirta; Bonassi, Stefano; Benedetti, Danieli; da Silva, Juliana; Toledo, Raphael; Salvadori, Daisy Maria Fávero; de Restrepo, Helena Groot; Filipic, Metka; Hercog, Klara; Aktaş, Ayça; Burgaz, Sema; Kundi, Michael; Grummt, Tamara; Thomas, Philip; Hor, Maryam; Escudero-Fung, Maria; Holland, Nina; Fenech, Michael.

In: Mutagenesis, 26.09.2016.

Research output: Contribution to journalArticle

Bolognesi, C, Knasmueller, S, Nersesyan, A, Roggieri, P, Ceppi, M, Bruzzone, M, Blaszczyk, E, Mielzynska-Svach, D, Milic, M, Bonassi, S, Benedetti, D, da Silva, J, Toledo, R, Salvadori, DMF, de Restrepo, HG, Filipic, M, Hercog, K, Aktaş, A, Burgaz, S, Kundi, M, Grummt, T, Thomas, P, Hor, M, Escudero-Fung, M, Holland, N & Fenech, M 2016, 'Inter-laboratory consistency and variability in the buccal micronucleus cytome assay depends on biomarker scored and laboratory experience: results from the HUMNxl international inter-laboratory scoring exercise', Mutagenesis. https://doi.org/10.1093/mutage/gew047
Bolognesi, Claudia ; Knasmueller, Siegfried ; Nersesyan, Armen ; Roggieri, Paola ; Ceppi, Marcello ; Bruzzone, Marco ; Blaszczyk, Ewa ; Mielzynska-Svach, Danuta ; Milic, Mirta ; Bonassi, Stefano ; Benedetti, Danieli ; da Silva, Juliana ; Toledo, Raphael ; Salvadori, Daisy Maria Fávero ; de Restrepo, Helena Groot ; Filipic, Metka ; Hercog, Klara ; Aktaş, Ayça ; Burgaz, Sema ; Kundi, Michael ; Grummt, Tamara ; Thomas, Philip ; Hor, Maryam ; Escudero-Fung, Maria ; Holland, Nina ; Fenech, Michael. / Inter-laboratory consistency and variability in the buccal micronucleus cytome assay depends on biomarker scored and laboratory experience : results from the HUMNxl international inter-laboratory scoring exercise. In: Mutagenesis. 2016.
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T1 - Inter-laboratory consistency and variability in the buccal micronucleus cytome assay depends on biomarker scored and laboratory experience

T2 - results from the HUMNxl international inter-laboratory scoring exercise

AU - Bolognesi, Claudia

AU - Knasmueller, Siegfried

AU - Nersesyan, Armen

AU - Roggieri, Paola

AU - Ceppi, Marcello

AU - Bruzzone, Marco

AU - Blaszczyk, Ewa

AU - Mielzynska-Svach, Danuta

AU - Milic, Mirta

AU - Bonassi, Stefano

AU - Benedetti, Danieli

AU - da Silva, Juliana

AU - Toledo, Raphael

AU - Salvadori, Daisy Maria Fávero

AU - de Restrepo, Helena Groot

AU - Filipic, Metka

AU - Hercog, Klara

AU - Aktaş, Ayça

AU - Burgaz, Sema

AU - Kundi, Michael

AU - Grummt, Tamara

AU - Thomas, Philip

AU - Hor, Maryam

AU - Escudero-Fung, Maria

AU - Holland, Nina

AU - Fenech, Michael

N1 - © The Author 2016. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2016/9/26

Y1 - 2016/9/26

N2 - The buccal micronucleus cytome (BMNcyt) assay in uncultured exfoliated epithelial cells from oral mucosa is widely applied in biomonitoring human exposures to genotoxic agents and is also proposed as a suitable test for prescreening and follow-up of precancerous oral lesions. The main limitation of the assay is the large variability observed in the baseline values of micronuclei (MNi) and other nuclear anomalies mainly related to different scoring criteria. The aim of this international collaborative study, involving laboratories with different level of experience, was to evaluate the inter- and intra-laboratory variations in the BMNcyt parameters, using recently implemented guidelines, in scoring cells from the same pooled samples obtained from healthy subjects (control group) and from cancer patients undergoing radiotherapy (treated group). The results indicate that all laboratories correctly discriminated samples from the two groups by a significant increase of micronucleus (MN) and nuclear bud (NBUD) frequencies and differentiated binucleated (BN) cells, associated with the exposure to ionizing radiation. The experience of the laboratories was shown to play an important role in the identification of the different cell types and nuclear anomalies. MN frequency in differentiated mononucleated (MONO) and BN cells showed the greatest consistency among the laboratories and low variability was also detected in the frequencies of MONO and BN cells. A larger variability was observed in classifying the different cell types, indicating the subjectivity in the interpretation of some of the scoring criteria while reproducibility of the results between scoring sessions was very good. An inter-laboratory calibration exercise is strongly recommended before starting studies with BMNcyt assay involving multiple research centers.

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