Interaction between cartilage oligomeric matrix protein and extracellular matrix protein 1 mediates endochondral bone growth

Li Kong, Qingyun Tian, Fengjin Guo, Maria T. Mucignat, Roberto Perris, Sandy Sercu, Joseph Merregaert, Paul E. Di Cesare, Chuan Ju Liu

Research output: Contribution to journalArticle

Abstract

In an effort to define the biological functions of COMP, a functional genetic screen was performed. This led to the identification of extracellular matrix protein 1 (ECM1) as a novel COMP-associated partner. COMP directly binds to ECM1 both in vitro and in vivo. The EGF domain of COMP and the C-terminus of ECM1 mediate the interaction between them. COMP and ECM1 colocalize in the growth plates in vivo. ECM1 inhibits chondrocyte hypertrophy, matrix mineralization, and endochondral bone formation, and COMP overcomes the inhibition by ECM1. In addition, COMP-mediated neutralization of ECM1 inhibition depends on their interaction, since COMP largely fails to overcome the ECM1 inhibition in the presence of the EGF domain of COMP, which disturbs the association of COMP and ECM1. These findings provide the first evidence linking the association of COMP and ECM1 and the biological significance underlying the interaction between them in regulating endochondral bone growth.

Original languageEnglish
Pages (from-to)276-286
Number of pages11
JournalMatrix Biology
Volume29
Issue number4
DOIs
Publication statusPublished - May 2010

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Keywords

  • Chondrocyte hypertrophy
  • COMP
  • ECM1
  • Endochondral bone growth
  • Matrix mineralization
  • Protein interaction

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Kong, L., Tian, Q., Guo, F., Mucignat, M. T., Perris, R., Sercu, S., Merregaert, J., Di Cesare, P. E., & Liu, C. J. (2010). Interaction between cartilage oligomeric matrix protein and extracellular matrix protein 1 mediates endochondral bone growth. Matrix Biology, 29(4), 276-286. https://doi.org/10.1016/j.matbio.2010.01.007