Interaction between cisplatin-modified DNA and the HMG boxes of HMG 1: DNase I footprinting and circular dichroism

D. Locker, M. Decoville, J. C. Maurizot, M. E. Bianchi, M. Leng

Research output: Contribution to journalArticlepeer-review

Abstract

The interactions between the two boxes A and B of HMG 1 and cis-diamminedichloroplatinum(II)-modified DNA containing a single intrastrand cross-link at the d(GpG) site were studied by DNase I footprinting and circular dichroism. The DNAase I cleavage patterns of the HMG box-platinated DNA complexes are identical, the two boxes inhibiting the DNase I cutting over at least 15 and 12 nucleotide residues in the platinated strand and the complementary strand, respectively. As judged by circular dichroism, the two boxes have the same α-helical content (56%) and they induce the same conformational changes in the platinated DNA.

Original languageEnglish
Pages (from-to)243-247
Number of pages5
JournalJournal of Molecular Biology
Volume246
Issue number2
DOIs
Publication statusPublished - 1995

Keywords

  • Cisplatin
  • Oligonucleotides
  • Transplatin

ASJC Scopus subject areas

  • Virology
  • Molecular Biology

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