Interaction between the cellular prion (PrPC) and the 2P domain K+ channel TREK-1 protein

Alberto Azzalin, Valentina Ferrara, Agustina Arias, Silvia Cerri, Debora Avella, Maria Bonaria Pisu, Rosanna Nano, Graziella Bernocchi, Luca Ferretti, Sergio Comincini

Research output: Contribution to journalArticlepeer-review

Abstract

The cellular prion protein (PrPC) is a highly conserved protein throughout the evolution of mammals and therefore is thought to play important cellular functions. Despite decades of intensive researches, the physiological function of PrPC remains enigmatic. Differently, in particular pathological contexts, generally referred as transmissible spongiform encephalopathies, a conformational isoform of PrPC, i.e., PrPSc, is considered the causative agent of these diseases. In this study, we investigated putative PrPC cellular functions through the identification of PrPC protein interactants. Using a bacterial two-hybrid approach, we identified a novel interaction between PrPC and a two-pore potassium channel protein, TREK-1. This interaction was further verified in transfected eukaryotic cells using co-immunoprecipitation and confocal microscopic analysis of the fluorescent transfected proteins. Importantly, in the cerebellar cortex, the endogenous PrPC and TREK-1 proteins exhibited co-localization signals in correspondence of the Purkinje cells. Furthermore, a deletion mapping study defined the carboxyl-terminal regions of the two proteins as the possible determinants of the PrPC-TREK-1 interaction. Our results indicated a novel PrPC interacting protein and suggested that this complex might be relevant in modulating a variety of electrophysiological-dependent cellular responses.

Original languageEnglish
Pages (from-to)108-115
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume346
Issue number1
DOIs
Publication statusPublished - Jul 21 2006

Keywords

  • Prion protein
  • Protein interaction
  • TREK-1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Interaction between the cellular prion (PrPC) and the 2P domain K+ channel TREK-1 protein'. Together they form a unique fingerprint.

Cite this