Interaction between tumor cell surface receptor RAGE and proteinase 3 mediates prostate cancer metastasis to bone

Mikhail G. Kolonin, Anna Sergeeva, Daniela I. Staquicini, Tracey L. Smith, Christy A. Tarleton, Jeffrey J. Molldrem, Richard L. Sidman, Serena Marchiò, Renata Pasqualini, Wadih Arap

Research output: Contribution to journalArticlepeer-review


Human prostate cancer often metastasizes to bone, but the biological basis for such site-specific tropism remains largely unresolved. Recent work led us to hypothesize that this tropism may reflect pathogenic interactions between RAGE, a cell surface receptor expressed on malignant cells in advanced prostate cancer, and proteinase 3 (PR3), a serine protease present in inflammatory neutrophils and hematopoietic cells within the bone marrow microenvironment. In this study, we establish that RAGE-PR3 interaction mediates homing of prostate cancer cells to the bone marrow. PR3 bound to RAGE on the surface of prostate cancer cells in vitro, inducing tumor cell motility through a nonproteolytic signal transduction cascade involving activation and phosphorylation of ERK1/2 and JNK1. In preclinical models of experimental metastasis, ectopic expression of RAGE on human prostate cancer cells was sufficient to promote bone marrow homing within a short timeframe. Our findings demonstrate how RAGE-PR3 interactions between human prostate cancer cells and the bone marrow microenvironment mediate bone metastasis during prostate cancer progression, with potential implications for prognosis and therapeutic intervention.

Original languageEnglish
Pages (from-to)3144-3150
Number of pages7
JournalCancer Research
Issue number12
Publication statusPublished - Jun 15 2017
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


Dive into the research topics of 'Interaction between tumor cell surface receptor RAGE and proteinase 3 mediates prostate cancer metastasis to bone'. Together they form a unique fingerprint.

Cite this