Interaction of 2'phosphoadenosine diphosphate ribose with erythrocytic 6-phosphogluconate dehydrogenase

C. Garre, R. Ravazzolo, F. Ajmar

Research output: Contribution to journalArticlepeer-review

Abstract

2' Phosphoadenosine Diphosphate Ribose specifically inactivates erythrocyte 6-Phosphogluconate Dehydrogenase with concomitant displacement of electrophoretic pattern and lowering of isoelectric point of the enzyme. 2'PADPR also shows competitive inhibition with respect to NADP+ and 6PG. Cibacron blue F3GA, the chromophore of blue dextran, is also a competitive inhibitor of the enzyme, the inhibition being mutually exclusive with 2'PADPR. A dinucleotide fold in 6-Phosphogluconate Dehydrogenase is suggested by Blue Dextran chromatography; the fold can be occupied by 2'PADPR, thus explaining enzyme inactivation.

Original languageEnglish
Pages (from-to)136-144
Number of pages9
JournalBulletin of Molecular Biology and Medicine
Volume2
Issue number3
Publication statusPublished - 1977

ASJC Scopus subject areas

  • Clinical Biochemistry

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