2' Phosphoadenosine Diphosphate Ribose specifically inactivates erythrocyte 6-Phosphogluconate Dehydrogenase with concomitant displacement of electrophoretic pattern and lowering of isoelectric point of the enzyme. 2'PADPR also shows competitive inhibition with respect to NADP+ and 6PG. Cibacron blue F3GA, the chromophore of blue dextran, is also a competitive inhibitor of the enzyme, the inhibition being mutually exclusive with 2'PADPR. A dinucleotide fold in 6-Phosphogluconate Dehydrogenase is suggested by Blue Dextran chromatography; the fold can be occupied by 2'PADPR, thus explaining enzyme inactivation.
|Number of pages||9|
|Journal||Bulletin of Molecular Biology and Medicine|
|Publication status||Published - 1977|
ASJC Scopus subject areas
- Clinical Biochemistry