Interaction of a protein from rat liver nuclei with cruciform DNA.

Research output: Contribution to journalArticle

Abstract

We constructed a synthetic cruciform DNA which closely resembles Holliday junctions, a DNA structure formed during recombination or following the transition from interstrand to intrastrand base pairing in palindromic DNA sequences. We identified and partially purified a protein from rat liver that specifically binds to this cruciform DNA molecule and does not bind to single-stranded or double-stranded DNAs of the same sequence. This protein also binds to the cruciform structure formed by a 70 bp palindromic sequence cloned in plasmid pUC18. No detectable nucleolytic activity is associated with the rat liver cruciform-binding protein, in contrast to all cruciform-recognizing proteins known so far.

Original languageEnglish
Pages (from-to)843-849
Number of pages7
JournalEMBO Journal
Volume7
Issue number3
Publication statusPublished - Mar 1988

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Cruciform DNA
Liver
Rats
DNA
Proteins
Base Pairing
Genetic Recombination
DNA sequences
Carrier Proteins
Plasmids
Molecules

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Interaction of a protein from rat liver nuclei with cruciform DNA. / Bianchi, M. E.

In: EMBO Journal, Vol. 7, No. 3, 03.1988, p. 843-849.

Research output: Contribution to journalArticle

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