Interaction of cefotetan and the metallo-β-lactamases produced in Aeromonas spp. and in vitro activity

M. I. Quiroga, N. Franceschini, G. M. Rossolini, G. Gutkind, G. Bonfiglio, L. Franchino, G. Amicosante

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aeromonas spp, are increasingly being recognized as human pathogens. The presence of metallo-β-lactamases in these organisms represents a potential problem in antimicrobial therapy. Mechanism-based inactivators of β-lactamases are used to overcome the resistance of clinical pathogens to β-lactam antibiotics, but no clinical useful inhibitors of the metallo-β-lactamases are presently known. Studying the interaction between cefotetan and Aeromonas spp. producing metallo-β-lactamase activity, we observed that cefotetan behaved as a transient inactivator for both the crude extracts of Aeromonas strains and the purified enzymes from Aeromonas hydrophila AE036 and Aeromonas schubertii MNSA20. The direct hydrolysis of cefotetan showed that it was a poor substrate for both purified enzymes. In view of the minimum inhibitory concentrations, cefotetan shows to be a useful antimicrobial agent against Aeromonas spp. Copyright (C) 2000 S. Karger AG. Basel.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalChemotherapy
Volume46
Issue number3
Publication statusPublished - 2000

Fingerprint

Cefotetan
Aeromonas
Aeromonas hydrophila
Lactams
Microbial Sensitivity Tests
Enzymes
Anti-Infective Agents
Complex Mixtures
Hydrolysis
In Vitro Techniques
Anti-Bacterial Agents

Keywords

  • Aeromonas metallo-β-lactamases
  • Cefotetan
  • Inhibition

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Quiroga, M. I., Franceschini, N., Rossolini, G. M., Gutkind, G., Bonfiglio, G., Franchino, L., & Amicosante, G. (2000). Interaction of cefotetan and the metallo-β-lactamases produced in Aeromonas spp. and in vitro activity. Chemotherapy, 46(3), 177-183.

Interaction of cefotetan and the metallo-β-lactamases produced in Aeromonas spp. and in vitro activity. / Quiroga, M. I.; Franceschini, N.; Rossolini, G. M.; Gutkind, G.; Bonfiglio, G.; Franchino, L.; Amicosante, G.

In: Chemotherapy, Vol. 46, No. 3, 2000, p. 177-183.

Research output: Contribution to journalArticle

Quiroga, MI, Franceschini, N, Rossolini, GM, Gutkind, G, Bonfiglio, G, Franchino, L & Amicosante, G 2000, 'Interaction of cefotetan and the metallo-β-lactamases produced in Aeromonas spp. and in vitro activity', Chemotherapy, vol. 46, no. 3, pp. 177-183.
Quiroga MI, Franceschini N, Rossolini GM, Gutkind G, Bonfiglio G, Franchino L et al. Interaction of cefotetan and the metallo-β-lactamases produced in Aeromonas spp. and in vitro activity. Chemotherapy. 2000;46(3):177-183.
Quiroga, M. I. ; Franceschini, N. ; Rossolini, G. M. ; Gutkind, G. ; Bonfiglio, G. ; Franchino, L. ; Amicosante, G. / Interaction of cefotetan and the metallo-β-lactamases produced in Aeromonas spp. and in vitro activity. In: Chemotherapy. 2000 ; Vol. 46, No. 3. pp. 177-183.
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