Interaction of DNA with cationic liposomes: Ability of transfecting lentil protoplasts

Mauro Maccarrone, Luciana Dini, Luisa Di Marzio, Antonio Di Giulio, Antonello Rossi, Giuseppe Mossa, Alessandro Finazzi-Agró

Research output: Contribution to journalArticle

Abstract

The vesicles made of dipalmitoylphosphatidylcholine and stearylamine (9:1) were multilamellar and rather homogeneous in shape as seen by transmission electron microscopy. Upon addition of circular DNA plasmids of different lengths to the liposomes, the formation of vesicle clusters around the DNA filament was observed, with dimensions dictated by the ratio DNA/lipid. These liposomes were able to transfect lentil (Lens culinaris) protoplasts inside the cells two different reporter genes, chloramphenicol-acetyltransferase and β-glucuronidase. The activity of these two enzymes could be found in the cell lysates after 24 h from the incubation of protoplasts with the lipid-DNA complexes.

Original languageEnglish
Pages (from-to)1417-1422
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number3
DOIs
Publication statusPublished - Aug 14 1992

Fingerprint

Lens Plant
Protoplasts
Liposomes
DNA
Lipids
1,2-Dipalmitoylphosphatidylcholine
Circular DNA
Chloramphenicol O-Acetyltransferase
Glucuronidase
Transmission Electron Microscopy
Reporter Genes
Lenses
Plasmids
Genes
Transmission electron microscopy
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Interaction of DNA with cationic liposomes : Ability of transfecting lentil protoplasts. / Maccarrone, Mauro; Dini, Luciana; Marzio, Luisa Di; Giulio, Antonio Di; Rossi, Antonello; Mossa, Giuseppe; Finazzi-Agró, Alessandro.

In: Biochemical and Biophysical Research Communications, Vol. 186, No. 3, 14.08.1992, p. 1417-1422.

Research output: Contribution to journalArticle

Maccarrone, Mauro ; Dini, Luciana ; Marzio, Luisa Di ; Giulio, Antonio Di ; Rossi, Antonello ; Mossa, Giuseppe ; Finazzi-Agró, Alessandro. / Interaction of DNA with cationic liposomes : Ability of transfecting lentil protoplasts. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 186, No. 3. pp. 1417-1422.
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