Interaction of human erythrocyte acetylcholinesterase with lectins

Roberto Ravazzolo; Cecilia Garrè; Franco Ajmar

doi:10.1080/00365518309168233

Interaction of human erythrocyte acetylcholinesterase with lectins

Roberto Ravazzolo, Cecilia Garrè, Franco Ajmar

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article

Abstract

Interaction of acetylcholinesterase (AChE) of human erythrocyte membranes with wheat germ lectin, Concanavalin A, Lens Culinaris lectin and phytohaemagglutinin is described. In solutions containing Triton X 100, only Wheat Germ lectin showed significant binding with AChE. Sepharose-immobilized Wheat Germ lectin bound AChE significantly more than Concanavalin A. This interaction was used for partial purification of the enzyme.

Original language	English
Pages (from-to)	123-126
Number of pages	4
Journal	Scandinavian Journal of Clinical and Laboratory Investigation
Volume	43
Issue number	2
DOIs	https://doi.org/10.1080/00365518309168233
Publication status	Published - 1983

Fingerprint

Keywords

Acetylcholinesterase
Sialic acid
Wheat germ lectin

ASJC Scopus subject areas

Clinical Biochemistry

Cite this

Ravazzolo, R., Garrè, C., & Ajmar, F. (1983). Interaction of human erythrocyte acetylcholinesterase with lectins. Scandinavian Journal of Clinical and Laboratory Investigation, 43(2), 123-126. https://doi.org/10.1080/00365518309168233

@article{385bb0ccdbc34259a5cef2988dfb74bc,

title = "Interaction of human erythrocyte acetylcholinesterase with lectins",

abstract = "Interaction of acetylcholinesterase (AChE) of human erythrocyte membranes with wheat germ lectin, Concanavalin A, Lens Culinaris lectin and phytohaemagglutinin is described. In solutions containing Triton X 100, only Wheat Germ lectin showed significant binding with AChE. Sepharose-immobilized Wheat Germ lectin bound AChE significantly more than Concanavalin A. This interaction was used for partial purification of the enzyme.",

keywords = "Acetylcholinesterase, Sialic acid, Wheat germ lectin",

author = "Roberto Ravazzolo and Cecilia Garr{\`e} and Franco Ajmar",

year = "1983",

doi = "10.1080/00365518309168233",

language = "English",

volume = "43",

pages = "123--126",

journal = "Scandinavian Journal of Clinical and Laboratory Investigation",

issn = "0036-5513",

publisher = "Informa Healthcare",

number = "2",

}

TY - JOUR

T1 - Interaction of human erythrocyte acetylcholinesterase with lectins

AU - Ravazzolo, Roberto

AU - Garrè, Cecilia

AU - Ajmar, Franco

PY - 1983

Y1 - 1983

N2 - Interaction of acetylcholinesterase (AChE) of human erythrocyte membranes with wheat germ lectin, Concanavalin A, Lens Culinaris lectin and phytohaemagglutinin is described. In solutions containing Triton X 100, only Wheat Germ lectin showed significant binding with AChE. Sepharose-immobilized Wheat Germ lectin bound AChE significantly more than Concanavalin A. This interaction was used for partial purification of the enzyme.

AB - Interaction of acetylcholinesterase (AChE) of human erythrocyte membranes with wheat germ lectin, Concanavalin A, Lens Culinaris lectin and phytohaemagglutinin is described. In solutions containing Triton X 100, only Wheat Germ lectin showed significant binding with AChE. Sepharose-immobilized Wheat Germ lectin bound AChE significantly more than Concanavalin A. This interaction was used for partial purification of the enzyme.

KW - Acetylcholinesterase

KW - Sialic acid

KW - Wheat germ lectin

UR - http://www.scopus.com/inward/record.url?scp=0020530886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020530886&partnerID=8YFLogxK

U2 - 10.1080/00365518309168233

DO - 10.1080/00365518309168233

M3 - Article

C2 - 6688478

AN - SCOPUS:0020530886

VL - 43

SP - 123

EP - 126

JO - Scandinavian Journal of Clinical and Laboratory Investigation

JF - Scandinavian Journal of Clinical and Laboratory Investigation

SN - 0036-5513

IS - 2

ER -

Access to Document

10.1080/00365518309168233