Interaction of human mesenchymal stem cells with cells involved in alloantigen-specific immune response favors the differentiation of CD4 + T-cell subsets expressing a regulatory/suppressive phenotype

Rita Maccario, Marina Podestà, Antonia Moretta, Angela Cometa, Patrizia Comoli, Daniela Montagna, Liane Daudt, Adalberto Ibatici, Giovanna Piaggio, Sarah Pozzi, Francesco Frassoni, Franco Locatelli

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Objectives. Experimental evidence and preliminary clinical studies have demonstrated that human mesenchymal stem cells (MSC) have an important immune modulatory function in the setting of allogeneic hematopoietic stem cell (HSC) H transplantation. We extended the evaluation of mechanisms responsible for the immune regulatory effect derived from the interaction of human MSC with cells involved in alloantigen-specific immune response in mixed lymphocyte culture (MLC). Design and Methods. Dendritic cell (DC) differentiation, T- and natural killer (NK)-lymphocyte expansion, alloantigen-specific cytotoxic activity and differentiation of CD4+ T-cell subsets co-expressing CD25 and/or CTLA4 molecules were assessed, comparing the effect observed using third-party MSC with that obtained employing MSC autologous to the MLC responder. Results. We found that human MSC strongly inhibit alloantigen-induced DC1 differentiation, down-regulate alloantigen-induced lymphocyte expansion, especially that of CD8+ T cells and of NK lymphocytes, decrease alloantigen-specific cytotoxic capacity mediated by either cytotoxic T lymphocytes or NK cells and favor the differentiation of CD4+ T-cell subsets co-expressing CD25 and/or CTLA4. More effective suppressive activity on MLC-induced T-cell activation was observed when MSC were third-party, rather than autologous, with respect to MLC-responder cells. Interpretation and Conclusions. Our results strongly suggest that MSC-mediated inhibition of alloantigen-induced DC1 differentiation and preferential activation of CD4+CD25+ T-cell subsets with presumed regulatory activity represent important mechanisms contributing to the immunosuppressive activity of MSC. Collectively, these data provide immunological support for the use of MSC to prevent immune complications related to both HSC and solid organ transplantation and to the theory that MSC are universal suppressors of immune reactivity.

Original languageEnglish
Pages (from-to)516-525
Number of pages10
JournalHaematologica
Volume90
Issue number4
Publication statusPublished - Apr 2005

Keywords

  • Dendritic cells (DC)
  • Mesenchymal stem cells (MSC)
  • Mixed lymphocyte culture (MLC)
  • Regulatory T-cells
  • Tolerance

ASJC Scopus subject areas

  • Hematology

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