Interaction of natural killer cells with extracellular matrix induces early intracellular signalling events and enhances cytotoxic functions

G. Palmieri, A. Gismondi, R. Galandrini, M. Milella, A. Serra, R. De Maria, A. Santoni

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

NK cells are endowed with a wide array of adhesion molecules which mediate their interaction with endothelium and extracellular matrix components. We have shown that cross-linking of β1 integrins and CD44 on human NK cells induces a signal transduction pathway involving both tyrosine kinase activation and the modulation of intracellular calcium levels. Our studies have also demonstrated the ability of β1 integrins and CD44 to upregulate both the spontaneous and the CD16-triggered cytotoxic activity of human NK cells. Although the molecular mechanisms responsible for this costimulatory activity have not been defined yet, our studies indicate that the simultaneous cross-linking of β1 integrins and CD44 results in a synergistic effect on CD16-mediated enhancement of intracellular free calcium concentration, and suggest that this may be relevant for the cooperation observed.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalNatural Immunity
Volume15
Issue number2-3
Publication statusPublished - Feb 1997

Fingerprint

Integrins
Natural Killer Cells
Extracellular Matrix
Calcium
Human Activities
Protein-Tyrosine Kinases
Endothelium
Signal Transduction
Up-Regulation

Keywords

  • Adhesion receptors
  • Cytotoxic function
  • Extracellular matrix
  • NK cells
  • Signal transduction

ASJC Scopus subject areas

  • Immunology

Cite this

Interaction of natural killer cells with extracellular matrix induces early intracellular signalling events and enhances cytotoxic functions. / Palmieri, G.; Gismondi, A.; Galandrini, R.; Milella, M.; Serra, A.; De Maria, R.; Santoni, A.

In: Natural Immunity, Vol. 15, No. 2-3, 02.1997, p. 147-153.

Research output: Contribution to journalArticle

@article{cf07a43daae846eb9d647a8021c435e0,
title = "Interaction of natural killer cells with extracellular matrix induces early intracellular signalling events and enhances cytotoxic functions",
abstract = "NK cells are endowed with a wide array of adhesion molecules which mediate their interaction with endothelium and extracellular matrix components. We have shown that cross-linking of β1 integrins and CD44 on human NK cells induces a signal transduction pathway involving both tyrosine kinase activation and the modulation of intracellular calcium levels. Our studies have also demonstrated the ability of β1 integrins and CD44 to upregulate both the spontaneous and the CD16-triggered cytotoxic activity of human NK cells. Although the molecular mechanisms responsible for this costimulatory activity have not been defined yet, our studies indicate that the simultaneous cross-linking of β1 integrins and CD44 results in a synergistic effect on CD16-mediated enhancement of intracellular free calcium concentration, and suggest that this may be relevant for the cooperation observed.",
keywords = "Adhesion receptors, Cytotoxic function, Extracellular matrix, NK cells, Signal transduction",
author = "G. Palmieri and A. Gismondi and R. Galandrini and M. Milella and A. Serra and {De Maria}, R. and A. Santoni",
year = "1997",
month = "2",
language = "English",
volume = "15",
pages = "147--153",
journal = "Natural Immunity",
issn = "1018-8916",
publisher = "S. Karger AG",
number = "2-3",

}

TY - JOUR

T1 - Interaction of natural killer cells with extracellular matrix induces early intracellular signalling events and enhances cytotoxic functions

AU - Palmieri, G.

AU - Gismondi, A.

AU - Galandrini, R.

AU - Milella, M.

AU - Serra, A.

AU - De Maria, R.

AU - Santoni, A.

PY - 1997/2

Y1 - 1997/2

N2 - NK cells are endowed with a wide array of adhesion molecules which mediate their interaction with endothelium and extracellular matrix components. We have shown that cross-linking of β1 integrins and CD44 on human NK cells induces a signal transduction pathway involving both tyrosine kinase activation and the modulation of intracellular calcium levels. Our studies have also demonstrated the ability of β1 integrins and CD44 to upregulate both the spontaneous and the CD16-triggered cytotoxic activity of human NK cells. Although the molecular mechanisms responsible for this costimulatory activity have not been defined yet, our studies indicate that the simultaneous cross-linking of β1 integrins and CD44 results in a synergistic effect on CD16-mediated enhancement of intracellular free calcium concentration, and suggest that this may be relevant for the cooperation observed.

AB - NK cells are endowed with a wide array of adhesion molecules which mediate their interaction with endothelium and extracellular matrix components. We have shown that cross-linking of β1 integrins and CD44 on human NK cells induces a signal transduction pathway involving both tyrosine kinase activation and the modulation of intracellular calcium levels. Our studies have also demonstrated the ability of β1 integrins and CD44 to upregulate both the spontaneous and the CD16-triggered cytotoxic activity of human NK cells. Although the molecular mechanisms responsible for this costimulatory activity have not been defined yet, our studies indicate that the simultaneous cross-linking of β1 integrins and CD44 results in a synergistic effect on CD16-mediated enhancement of intracellular free calcium concentration, and suggest that this may be relevant for the cooperation observed.

KW - Adhesion receptors

KW - Cytotoxic function

KW - Extracellular matrix

KW - NK cells

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=8044244197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8044244197&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 147

EP - 153

JO - Natural Immunity

JF - Natural Immunity

SN - 1018-8916

IS - 2-3

ER -