Interaction of rat liver glucocorticoid receptor with heparin

Alessandro Weisz, Giovanni Alfredo Puca, Maria Teresa Masucci, Carlo Masi, Rita Pagnotta, Antonella Petrillo, Vincenzo Sica

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

When rat liver cytosol containing [3H]dexamethasone-glucocorticoid receptor complex is exposed to immobilized heparin (Sepharose-heparin; Seph-hep) the steroid receptor complex binds to the substituted Sepharose avidly [Kd = 3.5 (±1.7) × 10-10 M], and 80-90% of the receptor present is adsorbed to the solid phase after 40 min at 0°C. The binding is enhanced by Mn2+ (10 mM) and Mg2+, whereas Ca2+ and Sr2+ are ineffective. Sodium molybdate (10 mM) does not influence the reaction but enhances receptor stability. Moreover, binding of the receptor to Seph-hep is dependent on the ionic strength of the medium, because binding is totally reversed by 300 mM KCl. The bound [3H]dexamethasone-receptor complex can be recovered from Seph-hep with solutions (4 mg/mL) of heparin (95% release), dextran sulfate (88%), and chondroitin sulfate (63%); total calf liver RNA is less effective (9%), whereas dextran, D-glucosamine, N-acetyl-D-glucosamine, D-glucuronic acid, and sheared calf thymus DNA are totally ineffective (3H] dexamethasone-receptor bound to the resin.

Original languageEnglish
Pages (from-to)5393-5397
Number of pages5
JournalBiochemistry
Volume23
Issue number23
Publication statusPublished - 1984

Fingerprint

Glucocorticoid Receptors
Liver
Heparin
Rats
Glucosamine
Glucuronic Acid
Dextran Sulfate
Acetylglucosamine
Chondroitin Sulfates
Steroid Receptors
Dextrans
Ionic strength
Sepharose
Dexamethasone
Resins
RNA
Cytosol
Osmolar Concentration
dexamethasone receptor
heparin-sepharose

ASJC Scopus subject areas

  • Biochemistry

Cite this

Weisz, A., Puca, G. A., Masucci, M. T., Masi, C., Pagnotta, R., Petrillo, A., & Sica, V. (1984). Interaction of rat liver glucocorticoid receptor with heparin. Biochemistry, 23(23), 5393-5397.

Interaction of rat liver glucocorticoid receptor with heparin. / Weisz, Alessandro; Puca, Giovanni Alfredo; Masucci, Maria Teresa; Masi, Carlo; Pagnotta, Rita; Petrillo, Antonella; Sica, Vincenzo.

In: Biochemistry, Vol. 23, No. 23, 1984, p. 5393-5397.

Research output: Contribution to journalArticle

Weisz, A, Puca, GA, Masucci, MT, Masi, C, Pagnotta, R, Petrillo, A & Sica, V 1984, 'Interaction of rat liver glucocorticoid receptor with heparin', Biochemistry, vol. 23, no. 23, pp. 5393-5397.
Weisz A, Puca GA, Masucci MT, Masi C, Pagnotta R, Petrillo A et al. Interaction of rat liver glucocorticoid receptor with heparin. Biochemistry. 1984;23(23):5393-5397.
Weisz, Alessandro ; Puca, Giovanni Alfredo ; Masucci, Maria Teresa ; Masi, Carlo ; Pagnotta, Rita ; Petrillo, Antonella ; Sica, Vincenzo. / Interaction of rat liver glucocorticoid receptor with heparin. In: Biochemistry. 1984 ; Vol. 23, No. 23. pp. 5393-5397.
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