Interaction of rat liver glucocorticoid receptor with heparin

Alessandro Weisz, Giovanni Alfredo Puca, Maria Teresa Masucci, Carlo Masi, Rita Pagnotta, Antonella Petrillo, Vincenzo Sica

Research output: Contribution to journalArticle

Abstract

When rat liver cytosol containing [3H]dexamethasone-glucocorticoid receptor complex is exposed to immobilized heparin (Sepharose-heparin; Seph-hep) the steroid receptor complex binds to the substituted Sepharose avidly [Kd = 3.5 (±1.7) × 10-10 M], and 80-90% of the receptor present is adsorbed to the solid phase after 40 min at 0°C. The binding is enhanced by Mn2+ (10 mM) and Mg2+, whereas Ca2+ and Sr2+ are ineffective. Sodium molybdate (10 mM) does not influence the reaction but enhances receptor stability. Moreover, binding of the receptor to Seph-hep is dependent on the ionic strength of the medium, because binding is totally reversed by 300 mM KCl. The bound [3H]dexamethasone-receptor complex can be recovered from Seph-hep with solutions (4 mg/mL) of heparin (95% release), dextran sulfate (88%), and chondroitin sulfate (63%); total calf liver RNA is less effective (9%), whereas dextran, D-glucosamine, N-acetyl-D-glucosamine, D-glucuronic acid, and sheared calf thymus DNA are totally ineffective (3H] dexamethasone-receptor bound to the resin.

Original languageEnglish
Pages (from-to)5393-5397
Number of pages5
JournalBiochemistry
Volume23
Issue number23
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Biochemistry

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    Weisz, A., Puca, G. A., Masucci, M. T., Masi, C., Pagnotta, R., Petrillo, A., & Sica, V. (1984). Interaction of rat liver glucocorticoid receptor with heparin. Biochemistry, 23(23), 5393-5397.