Interaction retinol-chromatin: an analysis of DNA from vitamin A-treated V79 Chinese hamster cells

Nicoletta Ferrari, Mattia Rocco, Anna Aprile, Gianfranco Fassina, Giorgio Vidali

Research output: Contribution to journalArticle

Abstract

When V79 cells are incubated in the presence of radiolabeled retinol, a small but consistent amount of radioactivity remains associated with nuclear DNA. Chromatographic analysis of enzymatic hydrolysates of DNA shows that no irreversible changes, such as adducts, have taken place on DNA. We present evidence that this radioactive incorporation may occur via a metabolic conversion of retinol, leading mainly to the formation of radiolabeled thymidine, which is then incorporated into newly made DNA. Mutagenic effects by retinol, given at concentrations well above physiological levels, have been also excluded.

Original languageEnglish
Pages (from-to)30-35
Number of pages6
JournalBBA - Gene Structure and Expression
Volume1007
Issue number1
DOIs
Publication statusPublished - Jan 13 1989

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Keywords

  • (V79 Chinese hamster cell line)
  • Chromatin-retinol interaction
  • DNA
  • Vitamin A

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

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