TY - JOUR
T1 - Interaction retinol-chromatin
T2 - an analysis of DNA from vitamin A-treated V79 Chinese hamster cells
AU - Ferrari, Nicoletta
AU - Rocco, Mattia
AU - Aprile, Anna
AU - Fassina, Gianfranco
AU - Vidali, Giorgio
PY - 1989/1/13
Y1 - 1989/1/13
N2 - When V79 cells are incubated in the presence of radiolabeled retinol, a small but consistent amount of radioactivity remains associated with nuclear DNA. Chromatographic analysis of enzymatic hydrolysates of DNA shows that no irreversible changes, such as adducts, have taken place on DNA. We present evidence that this radioactive incorporation may occur via a metabolic conversion of retinol, leading mainly to the formation of radiolabeled thymidine, which is then incorporated into newly made DNA. Mutagenic effects by retinol, given at concentrations well above physiological levels, have been also excluded.
AB - When V79 cells are incubated in the presence of radiolabeled retinol, a small but consistent amount of radioactivity remains associated with nuclear DNA. Chromatographic analysis of enzymatic hydrolysates of DNA shows that no irreversible changes, such as adducts, have taken place on DNA. We present evidence that this radioactive incorporation may occur via a metabolic conversion of retinol, leading mainly to the formation of radiolabeled thymidine, which is then incorporated into newly made DNA. Mutagenic effects by retinol, given at concentrations well above physiological levels, have been also excluded.
KW - (V79 Chinese hamster cell line)
KW - Chromatin-retinol interaction
KW - DNA
KW - Vitamin A
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U2 - 10.1016/0167-4781(89)90126-7
DO - 10.1016/0167-4781(89)90126-7
M3 - Article
C2 - 2535778
AN - SCOPUS:0024533098
VL - 1007
SP - 30
EP - 35
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -