Interaction retinol-chromatin: an analysis of DNA from vitamin A-treated V79 Chinese hamster cells

Nicoletta Ferrari; Mattia Rocco; Anna Aprile; Gianfranco Fassina; Giorgio Vidali

doi:10.1016/0167-4781(89)90126-7

Interaction retinol-chromatin: an analysis of DNA from vitamin A-treated V79 Chinese hamster cells

Nicoletta Ferrari, Mattia Rocco, Anna Aprile, Gianfranco Fassina, Giorgio Vidali

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article

Abstract

When V79 cells are incubated in the presence of radiolabeled retinol, a small but consistent amount of radioactivity remains associated with nuclear DNA. Chromatographic analysis of enzymatic hydrolysates of DNA shows that no irreversible changes, such as adducts, have taken place on DNA. We present evidence that this radioactive incorporation may occur via a metabolic conversion of retinol, leading mainly to the formation of radiolabeled thymidine, which is then incorporated into newly made DNA. Mutagenic effects by retinol, given at concentrations well above physiological levels, have been also excluded.

Original language	English
Pages (from-to)	30-35
Number of pages	6
Journal	BBA - Gene Structure and Expression
Volume	1007
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(89)90126-7
Publication status	Published - Jan 13 1989

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Keywords

(V79 Chinese hamster cell line)
Chromatin-retinol interaction
DNA
Vitamin A

ASJC Scopus subject areas

Biochemistry
Biophysics
Genetics
Structural Biology

Cite this

Ferrari, N., Rocco, M., Aprile, A., Fassina, G., & Vidali, G. (1989). Interaction retinol-chromatin: an analysis of DNA from vitamin A-treated V79 Chinese hamster cells. BBA - Gene Structure and Expression, 1007(1), 30-35. https://doi.org/10.1016/0167-4781(89)90126-7

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10.1016/0167-4781(89)90126-7